Quantitative microarray profiling provides evidence against widespread coupling of alternative splicing with nonsense-mediated mRNA decay to control gene expression

Qun Pan, Arneet L. Saltzman, Yoon Ki Kim, Christine Misquitta, Ofer Shai, Lynne E. Maquat, Brendan J. Frey, Benjamin J. Blencowe

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

Sequence-based analyses have predicted that ∼35% of mammalian alternative splicing (AS) events produce premature termination codon (PTC)-containing splice variants that are targeted by the process of nonsense-mediated mRNA decay (NMD). This led to speculation that AS may often regulate gene expression by activating NMD. Using AS microarrays, we show that PTC-containing splice variants are generally produced at uniformly low levels across diverse mammalian cells and tissues, independently of the action of NMD. Our results suggest that most PTC-introducing AS events are not under positive selection pressure and therefore may not contribute important functional roles.

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalGenes and Development
Volume20
Issue number2
DOIs
Publication statusPublished - 2006 Jan 15
Externally publishedYes

Fingerprint

Nonsense Mediated mRNA Decay
Alternative Splicing
Nonsense Codon
Gene Expression
Sequence Analysis
Pressure

Keywords

  • Alternative splicing
  • Microarray analysis
  • Nonsense-mediated mRNA decay
  • Premature termination codon

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

Quantitative microarray profiling provides evidence against widespread coupling of alternative splicing with nonsense-mediated mRNA decay to control gene expression. / Pan, Qun; Saltzman, Arneet L.; Kim, Yoon Ki; Misquitta, Christine; Shai, Ofer; Maquat, Lynne E.; Frey, Brendan J.; Blencowe, Benjamin J.

In: Genes and Development, Vol. 20, No. 2, 15.01.2006, p. 153-158.

Research output: Contribution to journalArticle

Pan, Qun ; Saltzman, Arneet L. ; Kim, Yoon Ki ; Misquitta, Christine ; Shai, Ofer ; Maquat, Lynne E. ; Frey, Brendan J. ; Blencowe, Benjamin J. / Quantitative microarray profiling provides evidence against widespread coupling of alternative splicing with nonsense-mediated mRNA decay to control gene expression. In: Genes and Development. 2006 ; Vol. 20, No. 2. pp. 153-158.
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