Quantitative microarray profiling provides evidence against widespread coupling of alternative splicing with nonsense-mediated mRNA decay to control gene expression

Qun Pan, Arneet L. Saltzman, Ki Kim Yoon, Christine Misquitta, Ofer Shai, Lynne E. Maquat, Brendan J. Frey, Benjamin J. Blencowe

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

Sequence-based analyses have predicted that ∼35% of mammalian alternative splicing (AS) events produce premature termination codon (PTC)-containing splice variants that are targeted by the process of nonsense-mediated mRNA decay (NMD). This led to speculation that AS may often regulate gene expression by activating NMD. Using AS microarrays, we show that PTC-containing splice variants are generally produced at uniformly low levels across diverse mammalian cells and tissues, independently of the action of NMD. Our results suggest that most PTC-introducing AS events are not under positive selection pressure and therefore may not contribute important functional roles.

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalGenes and Development
Volume20
Issue number2
DOIs
Publication statusPublished - 2006 Jan 15

Keywords

  • Alternative splicing
  • Microarray analysis
  • Nonsense-mediated mRNA decay
  • Premature termination codon

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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