Abstract
Sequence-based analyses have predicted that ∼35% of mammalian alternative splicing (AS) events produce premature termination codon (PTC)-containing splice variants that are targeted by the process of nonsense-mediated mRNA decay (NMD). This led to speculation that AS may often regulate gene expression by activating NMD. Using AS microarrays, we show that PTC-containing splice variants are generally produced at uniformly low levels across diverse mammalian cells and tissues, independently of the action of NMD. Our results suggest that most PTC-introducing AS events are not under positive selection pressure and therefore may not contribute important functional roles.
| Original language | English |
|---|---|
| Pages (from-to) | 153-158 |
| Number of pages | 6 |
| Journal | Genes and Development |
| Volume | 20 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2006 Jan 15 |
| Externally published | Yes |
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Keywords
- Alternative splicing
- Microarray analysis
- Nonsense-mediated mRNA decay
- Premature termination codon
ASJC Scopus subject areas
- Developmental Biology
- Genetics
Cite this
Quantitative microarray profiling provides evidence against widespread coupling of alternative splicing with nonsense-mediated mRNA decay to control gene expression. / Pan, Qun; Saltzman, Arneet L.; Kim, Yoon Ki; Misquitta, Christine; Shai, Ofer; Maquat, Lynne E.; Frey, Brendan J.; Blencowe, Benjamin J.
In: Genes and Development, Vol. 20, No. 2, 15.01.2006, p. 153-158.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitative microarray profiling provides evidence against widespread coupling of alternative splicing with nonsense-mediated mRNA decay to control gene expression
AU - Pan, Qun
AU - Saltzman, Arneet L.
AU - Kim, Yoon Ki
AU - Misquitta, Christine
AU - Shai, Ofer
AU - Maquat, Lynne E.
AU - Frey, Brendan J.
AU - Blencowe, Benjamin J.
PY - 2006/1/15
Y1 - 2006/1/15
N2 - Sequence-based analyses have predicted that ∼35% of mammalian alternative splicing (AS) events produce premature termination codon (PTC)-containing splice variants that are targeted by the process of nonsense-mediated mRNA decay (NMD). This led to speculation that AS may often regulate gene expression by activating NMD. Using AS microarrays, we show that PTC-containing splice variants are generally produced at uniformly low levels across diverse mammalian cells and tissues, independently of the action of NMD. Our results suggest that most PTC-introducing AS events are not under positive selection pressure and therefore may not contribute important functional roles.
AB - Sequence-based analyses have predicted that ∼35% of mammalian alternative splicing (AS) events produce premature termination codon (PTC)-containing splice variants that are targeted by the process of nonsense-mediated mRNA decay (NMD). This led to speculation that AS may often regulate gene expression by activating NMD. Using AS microarrays, we show that PTC-containing splice variants are generally produced at uniformly low levels across diverse mammalian cells and tissues, independently of the action of NMD. Our results suggest that most PTC-introducing AS events are not under positive selection pressure and therefore may not contribute important functional roles.
KW - Alternative splicing
KW - Microarray analysis
KW - Nonsense-mediated mRNA decay
KW - Premature termination codon
UR - http://www.scopus.com/inward/record.url?scp=30944449484&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=30944449484&partnerID=8YFLogxK
U2 - 10.1101/gad.1382806
DO - 10.1101/gad.1382806
M3 - Article
C2 - 16418482
AN - SCOPUS:30944449484
VL - 20
SP - 153
EP - 158
JO - Genes and Development
JF - Genes and Development
SN - 0890-9369
IS - 2
ER -