Quantitative phase imaging of live cells using fast Fourier phase microscopy

Niyom Lue, Wonshik Choi, Gabriel Popescu, Takahiro Ikeda, Ramachandra R. Dasari, Kamran Badizadegan, Michael S. Feld

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Using the decomposition of an image field in two spatial components that can be controllably shifted in phase with respect to each other, a new quantitative-phase microscope has been developed. The new instrument, referred to as the fast Fourier phase microscope (f-FPM), provides a factor of 100 higher acquisition rate compared with our previously reported Fourier phase microscope. The resulting quantitative-phase images are characterized by diffraction limited transverse resolution and path-length stability better than 2 nm at acquisition rates of 10 frames/s or more. These features make the f-FPM particularly appealing for investigating the structure and dynamics of live cells over a broad range of time scales. In addition, we demonstrate the possibility of examining subcellular structures by digitally processing the amplitude and phase information provided by the instrument. Thus we developed software that can emulate phase contrast and differential interference contrast microscopy images by numerically processing FPM images. This approach adds the flexibility of digitally varying the phase shift between the two interfering beams. The images obtained appear as if they were recorded by variable phase contrast or differential interference contrast microscopes that deliver an enhanced view to the subcellular structure when compared with the typical commercial microscope.

Original languageEnglish
Pages (from-to)1836-1842
Number of pages7
JournalApplied optics
Volume46
Issue number10
DOIs
Publication statusPublished - 2007 Apr 1
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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  • Cite this

    Lue, N., Choi, W., Popescu, G., Ikeda, T., Dasari, R. R., Badizadegan, K., & Feld, M. S. (2007). Quantitative phase imaging of live cells using fast Fourier phase microscopy. Applied optics, 46(10), 1836-1842. https://doi.org/10.1364/AO.46.001836