Quantitative profiling of plasma peptides in asthmatic mice using liquid chromatography and mass spectrometry

Sinkoo Yeo, Seob Roh Gu, Dong Hoon Kim, Min Lee Jung, Seong Won Seo, Won Cho Jin, Chan Wha Kim, KyuBum Kwack

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Asthma is increasing in prevalence worldwide as a result of factors associated with a Western lifestyle. However, simple and reliable diagnostic and prognostic markers are yet to be found. In an attempt to identify protein biomarker profiles among small molecular weight ranges, we employed an approach combining liquid chromatography with mass spectrometry, instead of two-dimensional gel electrophoresis (2-DE), which has previously been used to analyze protein expression patterns. Here we described its application to compare plasma peptides from control and chronic asthma mice. Peptides were quantitatively profiled as a multidimensional peptide mass fingerprint by a combination of reverse-phase high-performance liquid chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. They were identified by peptide mass fingerprinting using matrix-assisted laser desorption/ ionization time-of-flight/time-of-flight tandem mass spectrometry. In this study, we quantitatively identified the fragment f of complement 3 (C3f), which is important in inflammation. C3f was significantly higher in controls than chronic asthma mice. Our strategy allowed the detection and identification of different plasma peptides between control and chronic asthma mice on a proteomic scale. Therefore, these results suggest that native small peptides detected by non-2-DE techniques may be useful and specific biomarkers of disease.

Original languageEnglish
Pages (from-to)3308-3317
Number of pages10
JournalProteomics
Volume4
Issue number11
DOIs
Publication statusPublished - 2004 Nov 1

Fingerprint

Liquid chromatography
Liquid Chromatography
Mass spectrometry
Mass Spectrometry
Asthma
Plasmas
Peptides
Peptide Mapping
Lasers
Biomarkers
Complement C3
Ionization
Desorption
Electrophoresis, Gel, Two-Dimensional
Reverse-Phase Chromatography
Tandem Mass Spectrometry
Proteomics
Life Style
Proteins
Molecular Weight

Keywords

  • Asthma
  • Liquid chromatography
  • Mass spectrometry
  • Mice
  • Plasma

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yeo, S., Gu, S. R., Kim, D. H., Jung, M. L., Seo, S. W., Jin, W. C., ... Kwack, K. (2004). Quantitative profiling of plasma peptides in asthmatic mice using liquid chromatography and mass spectrometry. Proteomics, 4(11), 3308-3317. https://doi.org/10.1002/pmic.200400918

Quantitative profiling of plasma peptides in asthmatic mice using liquid chromatography and mass spectrometry. / Yeo, Sinkoo; Gu, Seob Roh; Kim, Dong Hoon; Jung, Min Lee; Seo, Seong Won; Jin, Won Cho; Kim, Chan Wha; Kwack, KyuBum.

In: Proteomics, Vol. 4, No. 11, 01.11.2004, p. 3308-3317.

Research output: Contribution to journalArticle

Yeo, Sinkoo ; Gu, Seob Roh ; Kim, Dong Hoon ; Jung, Min Lee ; Seo, Seong Won ; Jin, Won Cho ; Kim, Chan Wha ; Kwack, KyuBum. / Quantitative profiling of plasma peptides in asthmatic mice using liquid chromatography and mass spectrometry. In: Proteomics. 2004 ; Vol. 4, No. 11. pp. 3308-3317.
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