Quantitative proteome analysis of age-related changes in mouse gastrocnemius muscle using mTRAQ

Chae Young Hwang, Kyutae Kim, Jeong Yi Choi, Young Jae Bahn, Seung Min Lee, Yoon Ki Kim, Cheolju Lee, Ki Sun Kwon

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Aging is associated with a progressive loss of skeletal muscular function that often leads to progressive disability and loss of independence. Although muscle aging is well documented, the molecular mechanisms of this condition still remain unclear. To gain greater insight into the changes associated with aging of skeletal muscle, we performed quantitative proteomic analyses on young (6 months) and aged (27 months) mouse gastrocnemius muscles using mTRAQ stable isotope mass tags. We identified and quantified a total of 4585 peptides corresponding to 236 proteins (protein probability >0.9). Among them, 33 proteins were more than 1.5-fold upregulated and 20 proteins were more than 1.5-fold downregulated in aged muscle compared with young muscle. An ontological analysis revealed that differentially expressed proteins belonged to distinct functional groups, including ion homeostasis, energy metabolism, protein turnover, and Ca2+ signaling. Identified proteins included aralar1, β-enolase, fatty acid-binding protein 3, 3-hydroxyacyl-CoA dehydrogenase (Hadh), F-box protein 22, F-box, and leucine-rich repeat protein 18, voltage-dependent L-type calcium channel subunit beta-1, ryanodine receptor (RyR), and calsequestrin. Ectopic expression of calsequestrin in C2C12 myoblast resulted in decreased activity of nuclear factor of activated T-cells and increased levels of atrogin-1 and MuRF1 E3 ligase, suggesting that these differentially expressed proteins are involved in muscle aging.

Original languageEnglish
Pages (from-to)121-132
Number of pages12
JournalProteomics
Volume14
Issue number1
DOIs
Publication statusPublished - 2014 Jan 1

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Proteome
Muscle
Skeletal Muscle
Proteins
Aging of materials
Calsequestrin
Muscles
3-Hydroxyacyl-CoA Dehydrogenase
F-Box Proteins
NFATC Transcription Factors
Fatty Acid-Binding Proteins
L-Type Calcium Channels
Ryanodine Receptor Calcium Release Channel
Ubiquitin-Protein Ligases
Phosphopyruvate Hydratase
Myoblasts
Isotopes
Proteomics
Energy Metabolism
Functional groups

Keywords

  • Aging
  • Animal proteomics
  • Biomarker
  • MTRAQ
  • Skeletal muscle

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Hwang, C. Y., Kim, K., Choi, J. Y., Bahn, Y. J., Lee, S. M., Kim, Y. K., ... Kwon, K. S. (2014). Quantitative proteome analysis of age-related changes in mouse gastrocnemius muscle using mTRAQ. Proteomics, 14(1), 121-132. https://doi.org/10.1002/pmic.201200497

Quantitative proteome analysis of age-related changes in mouse gastrocnemius muscle using mTRAQ. / Hwang, Chae Young; Kim, Kyutae; Choi, Jeong Yi; Bahn, Young Jae; Lee, Seung Min; Kim, Yoon Ki; Lee, Cheolju; Kwon, Ki Sun.

In: Proteomics, Vol. 14, No. 1, 01.01.2014, p. 121-132.

Research output: Contribution to journalArticle

Hwang, CY, Kim, K, Choi, JY, Bahn, YJ, Lee, SM, Kim, YK, Lee, C & Kwon, KS 2014, 'Quantitative proteome analysis of age-related changes in mouse gastrocnemius muscle using mTRAQ', Proteomics, vol. 14, no. 1, pp. 121-132. https://doi.org/10.1002/pmic.201200497
Hwang, Chae Young ; Kim, Kyutae ; Choi, Jeong Yi ; Bahn, Young Jae ; Lee, Seung Min ; Kim, Yoon Ki ; Lee, Cheolju ; Kwon, Ki Sun. / Quantitative proteome analysis of age-related changes in mouse gastrocnemius muscle using mTRAQ. In: Proteomics. 2014 ; Vol. 14, No. 1. pp. 121-132.
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