Rab3a promotes brain tumor initiation and progression

Jun Kyum Kim, Seung Yup Lee, Chang Won Park, Suk Hwang Park, Jinlong Yin, Jaebong Kim, Jae Bong Park, Jae Yong Lee, Hyunggee Kim, Sung Chan Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The Rab protein family is composed of small GTP-binding proteins involved in intracellular vesicle trafficking. In particular, Rab3a which is one of four Rab3 proteins (a, b, c, and d isoforms) is associated with synaptic vesicle trafficking in normal brain. However, despite the elevated level of Rab3a in tumors, its role remains unclear. Here we report a tumorigenic role of Rab3a in brain tumors. Elevated level of Rab3a expression in human was confirmed in both glioma cell lines and glioblastoma multiforme patient specimens. Ectopic Rab3a expression in glioma cell lines and primary astrocytes promoted cell proliferation by increasing cyclin D1 expression, induced resistance to anti-cancer drug and irradiation, and accelerated foci formation in soft agar and tumor formation in nude mice. The overexpression of Rab3a augmented the tumorsphere-forming ability of glioma cells and p53-/- astrocytes and increased expression levels of various stem cell markers. Taken together, our results indicate that Rab3a is a novel oncogene involved in glioma initiation and progression.

Original languageEnglish
Pages (from-to)5903-5911
Number of pages9
JournalMolecular Biology Reports
Volume41
Issue number9
DOIs
Publication statusPublished - 2014 Jan 1

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ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Kim, J. K., Lee, S. Y., Park, C. W., Park, S. H., Yin, J., Kim, J., Park, J. B., Lee, J. Y., Kim, H., & Kim, S. C. (2014). Rab3a promotes brain tumor initiation and progression. Molecular Biology Reports, 41(9), 5903-5911. https://doi.org/10.1007/s11033-014-3465-2