Rac GTPase Activity is Essential for EGF-induced Mitogenesis

Byung Chul Kim, Ji Young Yi, Sun Ju Yi, In Cheol Shin, Kwon Soo Ha, Byung H. Jhun, Soon Bong Hwang, Jae-Hong Kim

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Rac, a member of the Rho family GTPases, has been implicated in the regulation of a wide range of biological processes including actin remodeling, cell transformation, G1 cell cycle progression, and gene expression. To determine whether Rac GTPase activity is required for epidermal growth factor-induced mitogenesis, Rat-2 stable cells expressing a dominant-negative Rac1 mutant, RacN17, were prepared. Exposure to EGF exhibited a significantly restricted growth response in Rat-2-RacN17 cells compared to Rat-2 parental cells, suggesting an essential role of Rac in EGF-induced mitogenesis. In contrast, addition of Iysophosphatidic acid exerted the same level of growth in Rat-2 and Rat-2-RacN17 cells. To gain further evidence for the essential role of Rac in EGF-induced mitogenesis, we performed the microinjection experiment. EGF-induced DNA synthesis was significantly blocked by microinjection of recombinant RacN17 protein, and not control IgG. Our further study to analyze the downstream mediator of Rac in EGF-signaling to mitogenesis demonstrated that Rac-activated phospholipase A2 plays a critical role. Taken together, our results suggest that the "Rac and Rac-activated PLA2" cascade is one of the major mitogenic pathways induced by EGF.

Original languageEnglish
Pages (from-to)90-95
Number of pages6
JournalMolecules and Cells
Volume8
Issue number1
Publication statusPublished - 1998 Feb 28
Externally publishedYes

Fingerprint

GTP Phosphohydrolases
Epidermal Growth Factor
Microinjections
Biological Phenomena
cdc Genes
rho GTP-Binding Proteins
Phospholipases A2
Growth
Recombinant Proteins
Actins
Immunoglobulin G
Gene Expression
Acids
DNA

Keywords

  • EGF
  • Mitogenesis
  • Phospholipase A
  • Rac

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Kim, B. C., Yi, J. Y., Yi, S. J., Shin, I. C., Ha, K. S., Jhun, B. H., ... Kim, J-H. (1998). Rac GTPase Activity is Essential for EGF-induced Mitogenesis. Molecules and Cells, 8(1), 90-95.

Rac GTPase Activity is Essential for EGF-induced Mitogenesis. / Kim, Byung Chul; Yi, Ji Young; Yi, Sun Ju; Shin, In Cheol; Ha, Kwon Soo; Jhun, Byung H.; Hwang, Soon Bong; Kim, Jae-Hong.

In: Molecules and Cells, Vol. 8, No. 1, 28.02.1998, p. 90-95.

Research output: Contribution to journalArticle

Kim, BC, Yi, JY, Yi, SJ, Shin, IC, Ha, KS, Jhun, BH, Hwang, SB & Kim, J-H 1998, 'Rac GTPase Activity is Essential for EGF-induced Mitogenesis', Molecules and Cells, vol. 8, no. 1, pp. 90-95.
Kim BC, Yi JY, Yi SJ, Shin IC, Ha KS, Jhun BH et al. Rac GTPase Activity is Essential for EGF-induced Mitogenesis. Molecules and Cells. 1998 Feb 28;8(1):90-95.
Kim, Byung Chul ; Yi, Ji Young ; Yi, Sun Ju ; Shin, In Cheol ; Ha, Kwon Soo ; Jhun, Byung H. ; Hwang, Soon Bong ; Kim, Jae-Hong. / Rac GTPase Activity is Essential for EGF-induced Mitogenesis. In: Molecules and Cells. 1998 ; Vol. 8, No. 1. pp. 90-95.
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