Rac1 contributes to maximal activation of STAT1 and STAT3 in IFN-γ-stimulated rat astrocytes

Eun Jung Park, Kyung Ae Ji, Sae Bom Jeon, Woo Hyuck Choi, Inn Oc Han, Hye Jin You, Jae Hong Kim, Ilo Jou, Eun Hye Joe

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Abstract

Rac1 GTPase is implicated as a signaling mediator in various cellular events. In this study, we show that Rac1 contributes to IFN-γ-induced inflammatory responses in rat astrocytes. We revealed that IFN-γ rapidly stimulated activation of Rac1 in C6 astroglioma cells by investigating GST-PAK-PBB-binding ability. We also found that Rac1 deficiency led to attenuation of IFN-γ-responsive transcriptional responses. Compared with levels in control cells, IFN-γ-induced IFN-γ-activated sequence promoter activity was markedly reduced in both C6 astroglioma cells and primary astrocytes expressing RacN17, a well-characterized Rac1-negative mutant. The expression of several IFN-γ-responsive genes, such as MCP-1 and ICAM-1, was also reduced in cells expressing RacN17. Consistent with these observations, IFN-γ-induced phosphorylation of STAT1 and STAT3 was lower in C6 cells expressing RacN17 (referred to as C6-RacN17) than in control cells. However, there was no difference in expression level of IFN-γRα subunit and IFN-γ-induced phosphorylation of JAK1 between C6 control and C6-RacN17 cells. Interestingly, Rac1 appeared to associate with IFN-γRα and augment the interaction of IFN-γR with either STAT1 or STAT3 in response to IFN-γ Taken together, we suggest that Rac1 may serve as an auxiliary mediator of IFN-γ-signaling, at least at the level of STAT activation, thus contributing to maximal activation of IFN-γ-responsive inflammatory signaling in rat astrocytes.

Original languageEnglish
Pages (from-to)5697-5703
Number of pages7
JournalJournal of Immunology
Volume173
Issue number9
Publication statusPublished - 2004 Nov 1

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Park, E. J., Ji, K. A., Jeon, S. B., Choi, W. H., Han, I. O., You, H. J., Kim, J. H., Jou, I., & Joe, E. H. (2004). Rac1 contributes to maximal activation of STAT1 and STAT3 in IFN-γ-stimulated rat astrocytes. Journal of Immunology, 173(9), 5697-5703.