Abstract
RanBPM is a scaffolding protein that regulates several cellular processes by interacting with various proteins. Previously, we reported that RanBPM acts as a negative regulator of BLT2, a low-affinity leukotriene B4 receptor; thus, it interferes with BLT2-mediated cell motility. In the present study, we observed that the expression levels of RanBPM were markedly reduced in the highly aggressive MDA-MB-435 and MDA-MB-231 human breast cancer cell lines compared with those in non-invasive MCF-7 cells. Additionally, we found that the restoration of RanBPM levels suppressed the invasiveness of these aggressive breast cancer cells in a manner dependent on BLT2 activation. In contrast, the knockdown of endogenous RanBPM by shRNA strongly promoted invasiveness in non-invasive MCF-7 cells. We also observed that RanBPM suppressed the invasiveness of aggressive breast cancer cells by inhibiting BLT2-mediated reactive oxygen species (ROS) generation and IL-8 production. Taken together, our results suggest that RanBPM acts as a negative regulator of BLT2, thus attenuating the invasiveness of aggressive breast cancer cells.
| Original language | English |
|---|---|
| Pages (from-to) | 305-311 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 483 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2017 Jan 29 |
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Keywords
- BLT2
- Breast cancer
- IL-8
- Invasiveness
- RanBPM
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
RanBPM inhibits BLT2-mediated IL-8 production and invasiveness in aggressive breast cancer cells. / Wei, Jun Dong; Jang, Jae Hyun; Kim, Jae-Hong.
In: Biochemical and Biophysical Research Communications, Vol. 483, No. 1, 29.01.2017, p. 305-311.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - RanBPM inhibits BLT2-mediated IL-8 production and invasiveness in aggressive breast cancer cells
AU - Wei, Jun Dong
AU - Jang, Jae Hyun
AU - Kim, Jae-Hong
PY - 2017/1/29
Y1 - 2017/1/29
N2 - RanBPM is a scaffolding protein that regulates several cellular processes by interacting with various proteins. Previously, we reported that RanBPM acts as a negative regulator of BLT2, a low-affinity leukotriene B4 receptor; thus, it interferes with BLT2-mediated cell motility. In the present study, we observed that the expression levels of RanBPM were markedly reduced in the highly aggressive MDA-MB-435 and MDA-MB-231 human breast cancer cell lines compared with those in non-invasive MCF-7 cells. Additionally, we found that the restoration of RanBPM levels suppressed the invasiveness of these aggressive breast cancer cells in a manner dependent on BLT2 activation. In contrast, the knockdown of endogenous RanBPM by shRNA strongly promoted invasiveness in non-invasive MCF-7 cells. We also observed that RanBPM suppressed the invasiveness of aggressive breast cancer cells by inhibiting BLT2-mediated reactive oxygen species (ROS) generation and IL-8 production. Taken together, our results suggest that RanBPM acts as a negative regulator of BLT2, thus attenuating the invasiveness of aggressive breast cancer cells.
AB - RanBPM is a scaffolding protein that regulates several cellular processes by interacting with various proteins. Previously, we reported that RanBPM acts as a negative regulator of BLT2, a low-affinity leukotriene B4 receptor; thus, it interferes with BLT2-mediated cell motility. In the present study, we observed that the expression levels of RanBPM were markedly reduced in the highly aggressive MDA-MB-435 and MDA-MB-231 human breast cancer cell lines compared with those in non-invasive MCF-7 cells. Additionally, we found that the restoration of RanBPM levels suppressed the invasiveness of these aggressive breast cancer cells in a manner dependent on BLT2 activation. In contrast, the knockdown of endogenous RanBPM by shRNA strongly promoted invasiveness in non-invasive MCF-7 cells. We also observed that RanBPM suppressed the invasiveness of aggressive breast cancer cells by inhibiting BLT2-mediated reactive oxygen species (ROS) generation and IL-8 production. Taken together, our results suggest that RanBPM acts as a negative regulator of BLT2, thus attenuating the invasiveness of aggressive breast cancer cells.
KW - BLT2
KW - Breast cancer
KW - IL-8
KW - Invasiveness
KW - RanBPM
UR - http://www.scopus.com/inward/record.url?scp=85009259968&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85009259968&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2016.12.147
DO - 10.1016/j.bbrc.2016.12.147
M3 - Article
C2 - 28027932
AN - SCOPUS:85009259968
VL - 483
SP - 305
EP - 311
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 1
ER -