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Translated title of the contribution: Rapid ABO genotyping using whole blood without DNA purification

Sung Ho Lee, Geon Park, Young Geun Yang, Seung Kwan Lee, Suhng Wook Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: ABO genotyping is commonly used in cases of an ABO discrepancy between cell typing and serum typing, as well as in forensic practice for personal identification and paternity testing. We evaluated ABO genotyping via multiplex allele-specific PCR (ASPCR) amplification using whole blood samples without DNA purification. Methods: A four-reaction multiplex ASPCR genotyping assay was designed to detect specific nucleotide sequence differences between the six ABO alleles A101, A102, B101, O01, O02, and cis-AB01. The ABO genotypes of 127 randomly chosen samples were determined using the new multiplex ASPCR method. Results: The genotypes of the 127 samples were found to be A101/A102 (n=1), A102/A102 (n=9), A101/O01 (n=3), A102/O01 (n=12), A102/O02 (n=14), B101/B101 (n=5), B101/O01 (n=18), B101/ O02 (n=15), O01/O01 (n=14), O02/O02 (n=8), O01/O02 (n=14) and A102/B101 (n=14), from which phenotypes were calculated to be A (n=39), B (n=38), O (n=36) and AB (n=14). The multiplex ASPCR assay results were compared with the serologically determined blood group phenotypes and genotypes determined by DNA sequencing, and there were no discrepancies. Conclusions: This convenient multiplex ASPCR assay, performed using whole blood samples, provides a supplement to routine serological ABO typing and might also be useful in other genotyping applications.

Translated title of the contributionRapid ABO genotyping using whole blood without DNA purification
Original languageKorean
Pages (from-to)231-237
Number of pages7
JournalKorean Journal of Laboratory Medicine
Volume29
Issue number3
DOIs
Publication statusPublished - 2009 Oct 12

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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