Rapid and Accurate Quantification of Paramylon Produced from Euglena gracilis Using an ssDNA Aptamer

Jee Young Kim, Jeong Joo Oh, Da Hee Kim, Jaewon Park, Hyun Soo Kim, Yoon E. Choi

Research output: Contribution to journalArticle

Abstract

The functional ingredients of microalgal biomass are receiving substantial recognition as the global demands for health supplements produced from natural sources are on the rise. Paramylon, a conglomerate of β-1,3-glucans, is one of the major valuable sources derived from Euglena gracilis having multiple applications, thus necessitating the development of an efficient quantification method. Here, we employed a DNA aptamer to quantify the amount of paramylon produced by E. gracilis. Paramylon-specific aptamers were isolated by the systematic evolution of ligands by exponential enrichment (SELEX) process. To evaluate the potential aptamers, the binding affinity between aptamer candidates and paramylon granules was confirmed by a confocal laser scanning microscope and the dissociation constants of the selected aptamers were determined by nonlinear regression analysis. The selected DNA aptamer was successfully used for the quantification of paramylon, and the results were compared to those obtained by the standard methods. The new approach was also used for quantification of paramylon from E. gracilis cells cultured to different cell stages and physiologies. It can be concluded that the aptamer-based protocol for the measurement of paramylon proposed in this study is highly accurate and comparatively less time-consuming.

Original languageEnglish
Pages (from-to)402-408
Number of pages7
JournalJournal of agricultural and food chemistry
Volume68
Issue number1
DOIs
Publication statusPublished - 2020 Jan 8

Fingerprint

Euglena gracilis
single-stranded DNA
Nucleotide Aptamers
DNA
glucans
microscopes
cultured cells
lasers
granules
regression analysis
ingredients
physiology
SELEX Aptamer Technique
biomass
Cell Physiological Phenomena
Glucans
methodology
Physiology
paramylon
Regression analysis

Keywords

  • DNA aptamer
  • Euglena gracilis
  • paramylon

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Rapid and Accurate Quantification of Paramylon Produced from Euglena gracilis Using an ssDNA Aptamer. / Kim, Jee Young; Oh, Jeong Joo; Kim, Da Hee; Park, Jaewon; Kim, Hyun Soo; Choi, Yoon E.

In: Journal of agricultural and food chemistry, Vol. 68, No. 1, 08.01.2020, p. 402-408.

Research output: Contribution to journalArticle

Kim, Jee Young ; Oh, Jeong Joo ; Kim, Da Hee ; Park, Jaewon ; Kim, Hyun Soo ; Choi, Yoon E. / Rapid and Accurate Quantification of Paramylon Produced from Euglena gracilis Using an ssDNA Aptamer. In: Journal of agricultural and food chemistry. 2020 ; Vol. 68, No. 1. pp. 402-408.
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