Rapid cell corpse clearance by stabilin-2, a membrane phosphatidylserine receptor

S. Y. Park, M. Y. Jung, H. J. Kim, S. J. Lee, S. Y. Kim, B. H. Lee, T. H. Kwon, R. W. Park, In-San Kim

Research output: Contribution to journalArticle

269 Citations (Scopus)

Abstract

Rapid phagocytic clearance of apoptotic cells is crucial for the prevention of both inflammation and autoimmune responses. Phosphatidylserine (PS) at the external surface of the plasma membrane has been proposed to function as a general 'eat me' signal for apoptotic cells. Although several soluble bridging molecules have been suggested for the recognition of PS, the PS-specific membrane receptor that binds directly to the exposed PS and provides a tickling signal has yet to be definitively identified. In this study, we provide evidence that stabilin-2 is a novel PS receptor, which performs a key function in the rapid clearance of cell corpses. It recognizes PS on aged red blood cells and apoptotic cells, and mediates their engulfment. The downregulation of stabilin-2 expression in macrophages significantly inhibits phagocytosis, and anti-stabilin-2 monoclonal antibody provokes the release of the anti-inflammatory cytokine, transforming growth factor-β. Furthermore, the results of time-lapse video analyses indicate that stabilin-2 performs a crucial function in the rapid clearance of aged and apoptotic cells. These data indicate that stabilin-2 is the first of the membrane PS receptors to provide tethering and tickling signals, and may also be involved in the resolution of inflammation and the prevention of autoimmunity.

Original languageEnglish
Pages (from-to)192-201
Number of pages10
JournalCell Death and Differentiation
Volume15
Issue number1
DOIs
Publication statusPublished - 2008 Jan 1
Externally publishedYes

Fingerprint

Phosphatidylserines
Cadaver
Membranes
Autoimmunity
Inflammation
Transforming Growth Factors
Phagocytosis
Anti-Inflammatory Agents
Down-Regulation
Erythrocytes
Macrophages
Monoclonal Antibodies
Cell Membrane
phosphatidylserine receptor
Cytokines

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Rapid cell corpse clearance by stabilin-2, a membrane phosphatidylserine receptor. / Park, S. Y.; Jung, M. Y.; Kim, H. J.; Lee, S. J.; Kim, S. Y.; Lee, B. H.; Kwon, T. H.; Park, R. W.; Kim, In-San.

In: Cell Death and Differentiation, Vol. 15, No. 1, 01.01.2008, p. 192-201.

Research output: Contribution to journalArticle

Park, SY, Jung, MY, Kim, HJ, Lee, SJ, Kim, SY, Lee, BH, Kwon, TH, Park, RW & Kim, I-S 2008, 'Rapid cell corpse clearance by stabilin-2, a membrane phosphatidylserine receptor', Cell Death and Differentiation, vol. 15, no. 1, pp. 192-201. https://doi.org/10.1038/sj.cdd.4402242
Park, S. Y. ; Jung, M. Y. ; Kim, H. J. ; Lee, S. J. ; Kim, S. Y. ; Lee, B. H. ; Kwon, T. H. ; Park, R. W. ; Kim, In-San. / Rapid cell corpse clearance by stabilin-2, a membrane phosphatidylserine receptor. In: Cell Death and Differentiation. 2008 ; Vol. 15, No. 1. pp. 192-201.
@article{3007e01f7f9e4bcfafc542c15c24f505,
title = "Rapid cell corpse clearance by stabilin-2, a membrane phosphatidylserine receptor",
abstract = "Rapid phagocytic clearance of apoptotic cells is crucial for the prevention of both inflammation and autoimmune responses. Phosphatidylserine (PS) at the external surface of the plasma membrane has been proposed to function as a general 'eat me' signal for apoptotic cells. Although several soluble bridging molecules have been suggested for the recognition of PS, the PS-specific membrane receptor that binds directly to the exposed PS and provides a tickling signal has yet to be definitively identified. In this study, we provide evidence that stabilin-2 is a novel PS receptor, which performs a key function in the rapid clearance of cell corpses. It recognizes PS on aged red blood cells and apoptotic cells, and mediates their engulfment. The downregulation of stabilin-2 expression in macrophages significantly inhibits phagocytosis, and anti-stabilin-2 monoclonal antibody provokes the release of the anti-inflammatory cytokine, transforming growth factor-β. Furthermore, the results of time-lapse video analyses indicate that stabilin-2 performs a crucial function in the rapid clearance of aged and apoptotic cells. These data indicate that stabilin-2 is the first of the membrane PS receptors to provide tethering and tickling signals, and may also be involved in the resolution of inflammation and the prevention of autoimmunity.",
author = "Park, {S. Y.} and Jung, {M. Y.} and Kim, {H. J.} and Lee, {S. J.} and Kim, {S. Y.} and Lee, {B. H.} and Kwon, {T. H.} and Park, {R. W.} and In-San Kim",
year = "2008",
month = "1",
day = "1",
doi = "10.1038/sj.cdd.4402242",
language = "English",
volume = "15",
pages = "192--201",
journal = "Cell Death and Differentiation",
issn = "1350-9047",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Rapid cell corpse clearance by stabilin-2, a membrane phosphatidylserine receptor

AU - Park, S. Y.

AU - Jung, M. Y.

AU - Kim, H. J.

AU - Lee, S. J.

AU - Kim, S. Y.

AU - Lee, B. H.

AU - Kwon, T. H.

AU - Park, R. W.

AU - Kim, In-San

PY - 2008/1/1

Y1 - 2008/1/1

N2 - Rapid phagocytic clearance of apoptotic cells is crucial for the prevention of both inflammation and autoimmune responses. Phosphatidylserine (PS) at the external surface of the plasma membrane has been proposed to function as a general 'eat me' signal for apoptotic cells. Although several soluble bridging molecules have been suggested for the recognition of PS, the PS-specific membrane receptor that binds directly to the exposed PS and provides a tickling signal has yet to be definitively identified. In this study, we provide evidence that stabilin-2 is a novel PS receptor, which performs a key function in the rapid clearance of cell corpses. It recognizes PS on aged red blood cells and apoptotic cells, and mediates their engulfment. The downregulation of stabilin-2 expression in macrophages significantly inhibits phagocytosis, and anti-stabilin-2 monoclonal antibody provokes the release of the anti-inflammatory cytokine, transforming growth factor-β. Furthermore, the results of time-lapse video analyses indicate that stabilin-2 performs a crucial function in the rapid clearance of aged and apoptotic cells. These data indicate that stabilin-2 is the first of the membrane PS receptors to provide tethering and tickling signals, and may also be involved in the resolution of inflammation and the prevention of autoimmunity.

AB - Rapid phagocytic clearance of apoptotic cells is crucial for the prevention of both inflammation and autoimmune responses. Phosphatidylserine (PS) at the external surface of the plasma membrane has been proposed to function as a general 'eat me' signal for apoptotic cells. Although several soluble bridging molecules have been suggested for the recognition of PS, the PS-specific membrane receptor that binds directly to the exposed PS and provides a tickling signal has yet to be definitively identified. In this study, we provide evidence that stabilin-2 is a novel PS receptor, which performs a key function in the rapid clearance of cell corpses. It recognizes PS on aged red blood cells and apoptotic cells, and mediates their engulfment. The downregulation of stabilin-2 expression in macrophages significantly inhibits phagocytosis, and anti-stabilin-2 monoclonal antibody provokes the release of the anti-inflammatory cytokine, transforming growth factor-β. Furthermore, the results of time-lapse video analyses indicate that stabilin-2 performs a crucial function in the rapid clearance of aged and apoptotic cells. These data indicate that stabilin-2 is the first of the membrane PS receptors to provide tethering and tickling signals, and may also be involved in the resolution of inflammation and the prevention of autoimmunity.

UR - http://www.scopus.com/inward/record.url?scp=37349029518&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=37349029518&partnerID=8YFLogxK

U2 - 10.1038/sj.cdd.4402242

DO - 10.1038/sj.cdd.4402242

M3 - Article

C2 - 17962816

AN - SCOPUS:37349029518

VL - 15

SP - 192

EP - 201

JO - Cell Death and Differentiation

JF - Cell Death and Differentiation

SN - 1350-9047

IS - 1

ER -