Rapid degradation of replication-dependent histone mRNAs largely occurs on mRNAs bound by nuclear cap-binding proteins 80 and 20

Junho Choe, Kyoung Mi Kim, Sungjin Park, Ye Kyung Lee, Ok Kyu Song, Min Kyung Kim, Byung Gil Lee, Hyun Kyu Song, Yoon Ki Kim

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The translation of mammalian messenger RNAs (mRNAs) can be driven by either cap-binding proteins 80 and 20 (CBP80/20) or eukaryotic translation initiation factor (eIF)4E. Although CBP80/20-dependent translation (CT) is known to be coupled to an mRNA surveillance mechanism termed nonsense-mediated mRNA decay (NMD), its molecular mechanism and biological role remain obscure. Here, using a yeast two-hybrid screening system, we identify a stem-loop binding protein (SLBP) that binds to a stem-loop structure at the 3′-end of the replication-dependent histone mRNA as a CT initiation factor (CTIF)-interacting protein. SLBP preferentially associates with the CT complex of histone mRNAs, but not with the eIF4E-depedent translation (ET) complex. Several lines of evidence indicate that rapid degradation of histone mRNA on the inhibition of DNA replication largely takes place during CT and not ET, which has been previously unappreciated. Furthermore, the ratio of CBP80/20-bound histone mRNA to eIF4E-bound histone mRNA is larger than the ratio of CBP80/20-bound polyadenylated β-actin or eEF2 mRNA to eIF4E-bound polyadenylated β-actin or eEF2 mRNA, respectively. The collective findings suggest that mRNAs harboring a different 3′-end use a different mechanism of translation initiation, expanding the repertoire of CT as a step for determining the fate of histone mRNAs.

Original languageEnglish
Pages (from-to)1307-1318
Number of pages12
JournalNucleic Acids Research
Volume41
Issue number2
DOIs
Publication statusPublished - 2013 Jan 1

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RNA Cap-Binding Proteins
Histones
Degradation
Messenger RNA
Actins
Carrier Proteins
Eukaryotic Initiation Factor-4E
Eukaryotic Initiation Factors
Peptide Initiation Factors
Two-Hybrid System Techniques
RNA Stability
DNA Replication
Yeast

ASJC Scopus subject areas

  • Genetics

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Rapid degradation of replication-dependent histone mRNAs largely occurs on mRNAs bound by nuclear cap-binding proteins 80 and 20. / Choe, Junho; Kim, Kyoung Mi; Park, Sungjin; Lee, Ye Kyung; Song, Ok Kyu; Kim, Min Kyung; Lee, Byung Gil; Song, Hyun Kyu; Kim, Yoon Ki.

In: Nucleic Acids Research, Vol. 41, No. 2, 01.01.2013, p. 1307-1318.

Research output: Contribution to journalArticle

Choe, Junho ; Kim, Kyoung Mi ; Park, Sungjin ; Lee, Ye Kyung ; Song, Ok Kyu ; Kim, Min Kyung ; Lee, Byung Gil ; Song, Hyun Kyu ; Kim, Yoon Ki. / Rapid degradation of replication-dependent histone mRNAs largely occurs on mRNAs bound by nuclear cap-binding proteins 80 and 20. In: Nucleic Acids Research. 2013 ; Vol. 41, No. 2. pp. 1307-1318.
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