Rapid determination of volatile organic compounds in human whole blood using static headspace sampling with gas chromatography and mass spectrometry

Ji Young Lee, Seungki Kim, Jong-Tae Lee, Jong-Ho Choi, Jeongae Lee, Heesoo Pyo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Headspace (HS) and headspace solid-phase microextraction (HS-SPME) were studied for extracting volatile organic compounds (VOCs) from whole blood, with chemical and instrumental variables being optimized for maximum sensitivity: incubation at 60 °C, equilibration for 30 min, pH 11, and 2 mL injection volume. Both techniques provided accurate analyses, with detection limits of 0.05-0.1 ng mL-1 and 0.05-0.5 ng mL-1. HS showed better sensitivity, reproducibility, and analysis times than HS-SPME. Overall levels of chloroform in whole blood were found to be 0.05-5.84 ng mL-1; detected levels of benzene were 0.05-2.20 ng mL-1.

Original languageEnglish
Pages (from-to)3963-3970
Number of pages8
JournalBulletin of the Korean Chemical Society
Volume33
Issue number12
DOIs
Publication statusPublished - 2012 Dec 20

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Volatile Organic Compounds
Gas chromatography
Mass spectrometry
Blood
Sampling
Chloroform
Benzene
4-sulfophenylmethallyl ether

Keywords

  • Headspace
  • HS-GC-MS
  • VOCs
  • Whole blood

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Rapid determination of volatile organic compounds in human whole blood using static headspace sampling with gas chromatography and mass spectrometry. / Lee, Ji Young; Kim, Seungki; Lee, Jong-Tae; Choi, Jong-Ho; Lee, Jeongae; Pyo, Heesoo.

In: Bulletin of the Korean Chemical Society, Vol. 33, No. 12, 20.12.2012, p. 3963-3970.

Research output: Contribution to journalArticle

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