Rapid determination of volatile organic compounds in human whole blood using static headspace sampling with gas chromatography and mass spectrometry

Ji Young Lee, Seungki Kim, Jong Tae Lee, Jong Ho Choi, Jeongae Lee, Heesoo Pyo

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Headspace (HS) and headspace solid-phase microextraction (HS-SPME) were studied for extracting volatile organic compounds (VOCs) from whole blood, with chemical and instrumental variables being optimized for maximum sensitivity: incubation at 60 °C, equilibration for 30 min, pH 11, and 2 mL injection volume. Both techniques provided accurate analyses, with detection limits of 0.05-0.1 ng mL-1 and 0.05-0.5 ng mL-1. HS showed better sensitivity, reproducibility, and analysis times than HS-SPME. Overall levels of chloroform in whole blood were found to be 0.05-5.84 ng mL-1; detected levels of benzene were 0.05-2.20 ng mL-1.

Original languageEnglish
Pages (from-to)3963-3970
Number of pages8
JournalBulletin of the Korean Chemical Society
Volume33
Issue number12
DOIs
Publication statusPublished - 2012 Dec 20

Keywords

  • HS-GC-MS
  • Headspace
  • VOCs
  • Whole blood

ASJC Scopus subject areas

  • Chemistry(all)

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