Rapid DNA hybridization analysis using a PDMS microfluidic sensor and a molecular beacon

Sungyong Kim, Lingxin Chen, Sangyeop Lee, Gi Hun Seong, Jaebum Choo, Eun Kyu Lee, Chil Hwan Oh, Sang Hoon Lee

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18 Citations (Scopus)

Abstract

A rapid DNA analysis has been developed based on a fluorescence intensity change of a molecular beacon in a PDMS microfluidic channel. Recently, we reported a new analytical method of DNA hybridization involving a PDMS microfluidic sensor using fluorescence energy transfer (FRET). However, there are some limitations in its application to real DNA samples because the target DNA must be labelled with a suitable fluorescent dye. To resolve this problem, we have developed a new DNA microfluidic sensor using a molecular beacon. By monitoring the change in the restored fluorescence intensity along the channel length, it is possible to rapidly detect any hybridization of the molecular beacon to the target DNA. In this case, the target DNA does not need to be labelled. Our experimental results demonstrate that this microfluidic sensor using a molecular beacon is a promising diagnostic tool for rapid DNA hybridization analysis. 2007

Original languageEnglish
Pages (from-to)401-405
Number of pages5
JournalAnalytical Sciences
Volume23
Issue number4
DOIs
Publication statusPublished - 2007 Apr 1

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ASJC Scopus subject areas

  • Analytical Chemistry

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