TY - JOUR
T1 - Rapid induction of gliogenesis in OLIG2 and NKX2.2-expressing progenitors-derived spheroids
AU - Yun, Wonjin
AU - Kim, In Yong
AU - Song, Gwonhwa
AU - You, Seungkwon
N1 - Funding Information:
This work was supported by the Bio & Medical Technology Development Program of the National Research Foundation of Korea, funded by the Korea Ministry of Science, ICT, & Future Planning (MSIP) (project no. NRF-2015M3A9B4071074), by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (Grant HI18C2166), by Korea University, by the Institute of Animal Molecular Biotechnology, and by the School of Life Sciences and Biotechnology for BK21 PLUS, Korea University, and STEMLAB, Inc.
PY - 2020/12
Y1 - 2020/12
N2 - Glial cells are crucial for the development of the central nervous system and the maintenance of chemical homeostasis. The process of gliogenesis has been well studied in the rodent brain, but it remains less well studied in the human brain. In addition, rodent glial cells differ from human counterparts in terms of morphologies, functions, and anatomical locations. Cerebral organoids (also referred to as spheroids) derived from human pluripotent stem cells (hPSCs) have been developed and are suitable cell-based models for researching developmental and neurodegenerative diseases. The in vitro generation of glia, including astrocytes and oligodendrocytes, from such organoids represents a promising tool to model neuronal diseases. Here, we showed that three-dimensional (3D) culture of OLIG2- and NKX2.2-expressing neurospheres produced efficiently mature astrocytes and oligodendrocytes in terms of morphologies and expression pattern recapitulating native 3D environment. Our findings provide important insights for developmental research of the human brain and glial specification that may facilitate patient-specific disease modeling.
AB - Glial cells are crucial for the development of the central nervous system and the maintenance of chemical homeostasis. The process of gliogenesis has been well studied in the rodent brain, but it remains less well studied in the human brain. In addition, rodent glial cells differ from human counterparts in terms of morphologies, functions, and anatomical locations. Cerebral organoids (also referred to as spheroids) derived from human pluripotent stem cells (hPSCs) have been developed and are suitable cell-based models for researching developmental and neurodegenerative diseases. The in vitro generation of glia, including astrocytes and oligodendrocytes, from such organoids represents a promising tool to model neuronal diseases. Here, we showed that three-dimensional (3D) culture of OLIG2- and NKX2.2-expressing neurospheres produced efficiently mature astrocytes and oligodendrocytes in terms of morphologies and expression pattern recapitulating native 3D environment. Our findings provide important insights for developmental research of the human brain and glial specification that may facilitate patient-specific disease modeling.
KW - drug target
KW - embryonic stem cells
KW - glia
KW - induced pluripotent stem cells
KW - oligodendrocytes
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U2 - 10.1002/sctm.19-0455
DO - 10.1002/sctm.19-0455
M3 - Article
AN - SCOPUS:85088571835
VL - 9
SP - 1643
EP - 1650
JO - Stem cells translational medicine
JF - Stem cells translational medicine
SN - 2157-6564
IS - 12
ER -