Abstract
A rapid method for the determination of anthocyanin glucosides and free delphinidin in food using ultra-high-performance liquid chromatography (u-HPLC) was validated through tests of precision, accuracy and linearity. The u-HPLC separation was performed on a reversed-phase C18 column (particle size 2 mm, i.d. 2 mm, length 100 mm) with a photodiode array detector. The limits of detection and quantification of the u-HPLC analyses ranged from 0.07 to 0.65 mg/kg for nine types of anthocyanin glucosides and from 0.08 to 0.26 mg/kg for delphinidin aglycone. The intra-day and inter-day precision of individual anthocyanin glucosides and delphinidin aglycone were less than 9.42%. All calibration curves showed good linearity (coefficient of determination 5 0.99) within the tested ranges. The rapid and simultaneous u-HPLC method presented in this study significantly improved the speed, sensitivity and resolution of the analyses of nine types of anthocyanin glucosides and free delphidinin aglycone in grapes.
| Original language | English |
|---|---|
| Pages (from-to) | 629-635 |
| Number of pages | 7 |
| Journal | Journal of Chromatographic Science |
| Volume | 52 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2014 Jan 1 |
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ASJC Scopus subject areas
- Analytical Chemistry
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Rapid method for determination of anthocyanin glucosides and free delphinidin in grapes using u-HPLC. / Shim, You Shin; Kim, Seunghee; Seo, Dongwon; Park, Hyun Jin; Ha, Jaeho.
In: Journal of Chromatographic Science, Vol. 52, No. 7, 01.01.2014, p. 629-635.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid method for determination of anthocyanin glucosides and free delphinidin in grapes using u-HPLC
AU - Shim, You Shin
AU - Kim, Seunghee
AU - Seo, Dongwon
AU - Park, Hyun Jin
AU - Ha, Jaeho
PY - 2014/1/1
Y1 - 2014/1/1
N2 - A rapid method for the determination of anthocyanin glucosides and free delphinidin in food using ultra-high-performance liquid chromatography (u-HPLC) was validated through tests of precision, accuracy and linearity. The u-HPLC separation was performed on a reversed-phase C18 column (particle size 2 mm, i.d. 2 mm, length 100 mm) with a photodiode array detector. The limits of detection and quantification of the u-HPLC analyses ranged from 0.07 to 0.65 mg/kg for nine types of anthocyanin glucosides and from 0.08 to 0.26 mg/kg for delphinidin aglycone. The intra-day and inter-day precision of individual anthocyanin glucosides and delphinidin aglycone were less than 9.42%. All calibration curves showed good linearity (coefficient of determination 5 0.99) within the tested ranges. The rapid and simultaneous u-HPLC method presented in this study significantly improved the speed, sensitivity and resolution of the analyses of nine types of anthocyanin glucosides and free delphidinin aglycone in grapes.
AB - A rapid method for the determination of anthocyanin glucosides and free delphinidin in food using ultra-high-performance liquid chromatography (u-HPLC) was validated through tests of precision, accuracy and linearity. The u-HPLC separation was performed on a reversed-phase C18 column (particle size 2 mm, i.d. 2 mm, length 100 mm) with a photodiode array detector. The limits of detection and quantification of the u-HPLC analyses ranged from 0.07 to 0.65 mg/kg for nine types of anthocyanin glucosides and from 0.08 to 0.26 mg/kg for delphinidin aglycone. The intra-day and inter-day precision of individual anthocyanin glucosides and delphinidin aglycone were less than 9.42%. All calibration curves showed good linearity (coefficient of determination 5 0.99) within the tested ranges. The rapid and simultaneous u-HPLC method presented in this study significantly improved the speed, sensitivity and resolution of the analyses of nine types of anthocyanin glucosides and free delphidinin aglycone in grapes.
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U2 - 10.1093/chromsci/bmt091
DO - 10.1093/chromsci/bmt091
M3 - Article
C2 - 23839806
AN - SCOPUS:84904334085
VL - 52
SP - 629
EP - 635
JO - Journal of Chromatographic Science
JF - Journal of Chromatographic Science
SN - 0021-9665
IS - 7
ER -