Rapid method for determination of anthocyanin glucosides and free delphinidin in grapes using u-HPLC

You Shin Shim, Seunghee Kim, Dongwon Seo, Hyun Jin Park, Jaeho Ha

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A rapid method for the determination of anthocyanin glucosides and free delphinidin in food using ultra-high-performance liquid chromatography (u-HPLC) was validated through tests of precision, accuracy and linearity. The u-HPLC separation was performed on a reversed-phase C18 column (particle size 2 mm, i.d. 2 mm, length 100 mm) with a photodiode array detector. The limits of detection and quantification of the u-HPLC analyses ranged from 0.07 to 0.65 mg/kg for nine types of anthocyanin glucosides and from 0.08 to 0.26 mg/kg for delphinidin aglycone. The intra-day and inter-day precision of individual anthocyanin glucosides and delphinidin aglycone were less than 9.42%. All calibration curves showed good linearity (coefficient of determination 5 0.99) within the tested ranges. The rapid and simultaneous u-HPLC method presented in this study significantly improved the speed, sensitivity and resolution of the analyses of nine types of anthocyanin glucosides and free delphidinin aglycone in grapes.

Original languageEnglish
Pages (from-to)629-635
Number of pages7
JournalJournal of Chromatographic Science
Volume52
Issue number7
DOIs
Publication statusPublished - 2014 Aug

ASJC Scopus subject areas

  • Analytical Chemistry

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