Rapid method for the determination of 14 isoflavones in food using UHPLC coupled to photo diode array detection

You Shin Shim, Won Jin Yoon, Jin Bong Hwang, Hyun Jin Park, Dongwon Seo, Jaeho Ha

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15 Citations (Scopus)


A rapid method for the determination of 14 types of isoflavones in food using ultra-high performance liquid chromatography (UHPLC) was validated in terms of precision, accuracy, sensitivity and linearity. The UHPLC separation was performed on a reverse-phase C18 column (particle size 2 μm, i.d. 2 mm, length 100 mm) using a photo diode array detector that was fixed to 260 nm. The limits of detection and quantification of the UHPLC analyses ranged from 0.03 to 0.33 mg kg<sup>-1</sup>. The intra-day and inter-day precision of the individual isoflavones were less than 11.77% and calibration curves exhibited good linearity (r<sup>2</sup> = 0.99) within the tested ranges. These results suggest that the rapid method used in this study could be available to determine of 14 types of isoflavones in a variety of food such as soy bean, black bean, red bean and soybean paste.

Original languageEnglish
Pages (from-to)391-397
Number of pages7
JournalFood Chemistry
Publication statusPublished - 2015 Nov 15



  • Isoflavone
  • Method validation
  • Rapid method

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

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