Rapid method for the determination of 14 isoflavones in food using UHPLC coupled to photo diode array detection

You Shin Shim, Won Jin Yoon, Jin Bong Hwang, Hyun Jin Park, Dongwon Seo, Jaeho Ha

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A rapid method for the determination of 14 types of isoflavones in food using ultra-high performance liquid chromatography (UHPLC) was validated in terms of precision, accuracy, sensitivity and linearity. The UHPLC separation was performed on a reverse-phase C18 column (particle size 2 μm, i.d. 2 mm, length 100 mm) using a photo diode array detector that was fixed to 260 nm. The limits of detection and quantification of the UHPLC analyses ranged from 0.03 to 0.33 mg kg<sup>-1</sup>. The intra-day and inter-day precision of the individual isoflavones were less than 11.77% and calibration curves exhibited good linearity (r<sup>2</sup> = 0.99) within the tested ranges. These results suggest that the rapid method used in this study could be available to determine of 14 types of isoflavones in a variety of food such as soy bean, black bean, red bean and soybean paste.

Original languageEnglish
Pages (from-to)391-397
Number of pages7
JournalFood Chemistry
Volume187
DOIs
Publication statusPublished - 2015 Nov 15

Fingerprint

ultra-performance liquid chromatography
Isoflavones
High performance liquid chromatography
isoflavones
rapid methods
Diodes
High Pressure Liquid Chromatography
Soybeans
Food
soybeans
red beans
black beans
Ointments
Particle Size
Calibration
detectors
Limit of Detection
particle size
detection limit
foods

Keywords

  • Isoflavone
  • Method validation
  • Rapid method
  • UHPLC

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Rapid method for the determination of 14 isoflavones in food using UHPLC coupled to photo diode array detection. / Shim, You Shin; Yoon, Won Jin; Hwang, Jin Bong; Park, Hyun Jin; Seo, Dongwon; Ha, Jaeho.

In: Food Chemistry, Vol. 187, 15.11.2015, p. 391-397.

Research output: Contribution to journalArticle

Shim, You Shin ; Yoon, Won Jin ; Hwang, Jin Bong ; Park, Hyun Jin ; Seo, Dongwon ; Ha, Jaeho. / Rapid method for the determination of 14 isoflavones in food using UHPLC coupled to photo diode array detection. In: Food Chemistry. 2015 ; Vol. 187. pp. 391-397.
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