Abstract
A rapid method for the simultaneous determination of flavonol aglycones in food using ultrahigh-performance LC (u-HPLC) coupled with a heating-block acidic hydrolysis method was validated in terms of precision, accuracy, and linearity. The u-HPLC separation was performed on an RP C18 column (particle size 2 μm id, 2 mm, length 100 mm) with a photodiode array detector. The LOD and LOQ of the u-HPLC analyses were 0.15 and 0.47 mg/kg for myricetin, 0.09 and 0.28 mg/kg for quercetin, 0.16 and 0.49 mg/kg for kaempferol, and 0.08 and 0.25 mg/kg for isorhamnetin. The intraday and interday precisions of the individual flavonol aglycones were less than 9.31%. All calibration curves exhibited good linearity (r2 = 0.99) within the tested ranges. Total run time of u-HPLC was 13 min. The rapid u-HPLC method presented herein significantly improved the speed, sensitivity, and resolution of the analyses of myricetin, quercetin, kaempferol, and isorhamnetin in food.
| Original language | English |
|---|---|
| Pages (from-to) | 1059-1064 |
| Number of pages | 6 |
| Journal | Journal of AOAC International |
| Volume | 96 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2013 Sep 1 |
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ASJC Scopus subject areas
- Analytical Chemistry
- Agronomy and Crop Science
- Food Science
- Environmental Chemistry
- Pharmacology
Cite this
Rapid method for the simultaneous determination of flavonol aglycones in food using u-HPLC coupled with heating block acidic hydrolysis. / Shim, You Shin; Kim, Seunghee; Seo, Dongwon; Ito, Masahito; Nakagawa, Hiroaki; Park, Hyun Jin; Ha, Jaeho.
In: Journal of AOAC International, Vol. 96, No. 5, 01.09.2013, p. 1059-1064.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid method for the simultaneous determination of flavonol aglycones in food using u-HPLC coupled with heating block acidic hydrolysis
AU - Shim, You Shin
AU - Kim, Seunghee
AU - Seo, Dongwon
AU - Ito, Masahito
AU - Nakagawa, Hiroaki
AU - Park, Hyun Jin
AU - Ha, Jaeho
PY - 2013/9/1
Y1 - 2013/9/1
N2 - A rapid method for the simultaneous determination of flavonol aglycones in food using ultrahigh-performance LC (u-HPLC) coupled with a heating-block acidic hydrolysis method was validated in terms of precision, accuracy, and linearity. The u-HPLC separation was performed on an RP C18 column (particle size 2 μm id, 2 mm, length 100 mm) with a photodiode array detector. The LOD and LOQ of the u-HPLC analyses were 0.15 and 0.47 mg/kg for myricetin, 0.09 and 0.28 mg/kg for quercetin, 0.16 and 0.49 mg/kg for kaempferol, and 0.08 and 0.25 mg/kg for isorhamnetin. The intraday and interday precisions of the individual flavonol aglycones were less than 9.31%. All calibration curves exhibited good linearity (r2 = 0.99) within the tested ranges. Total run time of u-HPLC was 13 min. The rapid u-HPLC method presented herein significantly improved the speed, sensitivity, and resolution of the analyses of myricetin, quercetin, kaempferol, and isorhamnetin in food.
AB - A rapid method for the simultaneous determination of flavonol aglycones in food using ultrahigh-performance LC (u-HPLC) coupled with a heating-block acidic hydrolysis method was validated in terms of precision, accuracy, and linearity. The u-HPLC separation was performed on an RP C18 column (particle size 2 μm id, 2 mm, length 100 mm) with a photodiode array detector. The LOD and LOQ of the u-HPLC analyses were 0.15 and 0.47 mg/kg for myricetin, 0.09 and 0.28 mg/kg for quercetin, 0.16 and 0.49 mg/kg for kaempferol, and 0.08 and 0.25 mg/kg for isorhamnetin. The intraday and interday precisions of the individual flavonol aglycones were less than 9.31%. All calibration curves exhibited good linearity (r2 = 0.99) within the tested ranges. Total run time of u-HPLC was 13 min. The rapid u-HPLC method presented herein significantly improved the speed, sensitivity, and resolution of the analyses of myricetin, quercetin, kaempferol, and isorhamnetin in food.
UR - http://www.scopus.com/inward/record.url?scp=84892187858&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84892187858&partnerID=8YFLogxK
U2 - 10.5740/jaoacint.12-439
DO - 10.5740/jaoacint.12-439
M3 - Article
C2 - 24282947
AN - SCOPUS:84892187858
VL - 96
SP - 1059
EP - 1064
JO - Journal of AOAC International
JF - Journal of AOAC International
SN - 1060-3271
IS - 5
ER -