Abstract
A rapid method for the simultaneous determination of flavonol aglycones in food using ultrahigh-performance LC (u-HPLC) coupled with a heating-block acidic hydrolysis method was validated in terms of precision, accuracy, and linearity. The u-HPLC separation was performed on an RP C18 column (particle size 2 μm id, 2 mm, length 100 mm) with a photodiode array detector. The LOD and LOQ of the u-HPLC analyses were 0.15 and 0.47 mg/kg for myricetin, 0.09 and 0.28 mg/kg for quercetin, 0.16 and 0.49 mg/kg for kaempferol, and 0.08 and 0.25 mg/kg for isorhamnetin. The intraday and interday precisions of the individual flavonol aglycones were less than 9.31%. All calibration curves exhibited good linearity (r2 = 0.99) within the tested ranges. Total run time of u-HPLC was 13 min. The rapid u-HPLC method presented herein significantly improved the speed, sensitivity, and resolution of the analyses of myricetin, quercetin, kaempferol, and isorhamnetin in food.
Original language | English |
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Pages (from-to) | 1059-1064 |
Number of pages | 6 |
Journal | Journal of AOAC International |
Volume | 96 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2013 Sep |
ASJC Scopus subject areas
- Analytical Chemistry
- Food Science
- Environmental Chemistry
- Agronomy and Crop Science
- Pharmacology