Abstract
There has been an urgent need to quickly screen and isolate patients with viral infections from patients with similar symptoms at point-of-care. In this study, we introduce a new microfluidic method for detection of various viruses using rolling circle amplification (RCA) of pathogens on the surface of thousands of microbeads packed in microchannels. When a targeted pathogen meets the corresponding particular template, the DNAs are rapidly amplified into a specific dumbbell shape through the RCA process, forming a DNA hydrogel and blocking the flow path formed between the beads. Due to the significant increase in reaction surface area, the detection time was shortened to less than 15 min and the detection limit of various pathogens has been reached to 0.1 pM. By injecting the stained liquid, the existence of the target pathogens in a sample fluid can be determined with the naked eye. Furthermore, by integrating multi-channel design, simultaneous phenotyping of various infective pathogens (i.e., Ebola, Middle East respiratory syndrome (MERS), and others) in biological specimens can be performed at a point-of-care.
| Original language | English |
|---|---|
| Pages (from-to) | 9-13 |
| Number of pages | 5 |
| Journal | Biosensors and Bioelectronics |
| Volume | 108 |
| DOIs | |
| Publication status | Published - 2018 Jun 15 |
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Keywords
- DNA
- Hydrogel
- Microbead
- Microfluidics
- Molecular diagnosis
- RCA
ASJC Scopus subject areas
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry
Cite this
Rapid molecular diagnosis of infectious viruses in microfluidics using DNA hydrogel formation. / Na, Wonhwi; Nam, Dongwoo; Lee, Hoyoon; Shin, Sehyun.
In: Biosensors and Bioelectronics, Vol. 108, 15.06.2018, p. 9-13.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid molecular diagnosis of infectious viruses in microfluidics using DNA hydrogel formation
AU - Na, Wonhwi
AU - Nam, Dongwoo
AU - Lee, Hoyoon
AU - Shin, Sehyun
PY - 2018/6/15
Y1 - 2018/6/15
N2 - There has been an urgent need to quickly screen and isolate patients with viral infections from patients with similar symptoms at point-of-care. In this study, we introduce a new microfluidic method for detection of various viruses using rolling circle amplification (RCA) of pathogens on the surface of thousands of microbeads packed in microchannels. When a targeted pathogen meets the corresponding particular template, the DNAs are rapidly amplified into a specific dumbbell shape through the RCA process, forming a DNA hydrogel and blocking the flow path formed between the beads. Due to the significant increase in reaction surface area, the detection time was shortened to less than 15 min and the detection limit of various pathogens has been reached to 0.1 pM. By injecting the stained liquid, the existence of the target pathogens in a sample fluid can be determined with the naked eye. Furthermore, by integrating multi-channel design, simultaneous phenotyping of various infective pathogens (i.e., Ebola, Middle East respiratory syndrome (MERS), and others) in biological specimens can be performed at a point-of-care.
AB - There has been an urgent need to quickly screen and isolate patients with viral infections from patients with similar symptoms at point-of-care. In this study, we introduce a new microfluidic method for detection of various viruses using rolling circle amplification (RCA) of pathogens on the surface of thousands of microbeads packed in microchannels. When a targeted pathogen meets the corresponding particular template, the DNAs are rapidly amplified into a specific dumbbell shape through the RCA process, forming a DNA hydrogel and blocking the flow path formed between the beads. Due to the significant increase in reaction surface area, the detection time was shortened to less than 15 min and the detection limit of various pathogens has been reached to 0.1 pM. By injecting the stained liquid, the existence of the target pathogens in a sample fluid can be determined with the naked eye. Furthermore, by integrating multi-channel design, simultaneous phenotyping of various infective pathogens (i.e., Ebola, Middle East respiratory syndrome (MERS), and others) in biological specimens can be performed at a point-of-care.
KW - DNA
KW - Hydrogel
KW - Microbead
KW - Microfluidics
KW - Molecular diagnosis
KW - RCA
UR - http://www.scopus.com/inward/record.url?scp=85042420724&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85042420724&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2018.02.040
DO - 10.1016/j.bios.2018.02.040
M3 - Article
C2 - 29494886
AN - SCOPUS:85042420724
VL - 108
SP - 9
EP - 13
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
ER -