Rapid quantification of cellular flavonoid levels using quercetin and a fluorescent diphenylboric acid 2-amino ethyl ester probe

Ji Hae Lee, Yeojin Kim, Minh Hien Hoang, Hee jin Jun, Sung-Joon Lee

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Flavonoids are abundant in fruits and vegetables and have various biological activities. A rapid, sensitive method for assessing cellular quercetin levels is necessary because cellular levels are often low. A new method for measuring cellular flavonoid levels is proposed using diphenylboric acid 2-aminoethyl ester (DPBA), a fluorescent probe, and quercetin, a representative flavonoid. This spectrofluorometry-based assay is simple and allows rapid quantification of the cellular quercetin content with high sensitivity. CHO-K1 cells were incubated with quercetin for 5 min, and then the cellular quercetin concentration was assayed using DPBA spectrofluorometry. Results, compared with high-performance liquid chromatography (HPLC), thin layer chromatography (TLC), and conventional spectrophotometry measurements, were highly correlated with HPLC data (R>0.99). The method is more sensitive than TLC and conventional spectrophotometry. Spectrofluorometric measurement using DPBA is a simple, rapid, and sensitive method for assessing cellular quercetin levels.

Original languageEnglish
Pages (from-to)75-79
Number of pages5
JournalFood Science and Biotechnology
Volume23
Issue number1
DOIs
Publication statusPublished - 2014 Feb 1

Fingerprint

Quercetin
Flavonoids
quercetin
Esters
flavonoids
esters
Amino Acids
acids
fluorescence emission spectroscopy
Spectrophotometry
Fluorescence Spectrometry
Thin Layer Chromatography
thin layer chromatography
Acids
spectroscopy
high performance liquid chromatography
High Pressure Liquid Chromatography
CHO Cells
methodology
Fluorescent Dyes

Keywords

  • diphenylboric acid 2-aminoethyl ether (DPBA)
  • flavonoids
  • fluorescence activated cell sorter (FACS)
  • quercetin
  • spectrofluorometry

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Rapid quantification of cellular flavonoid levels using quercetin and a fluorescent diphenylboric acid 2-amino ethyl ester probe. / Lee, Ji Hae; Kim, Yeojin; Hoang, Minh Hien; Jun, Hee jin; Lee, Sung-Joon.

In: Food Science and Biotechnology, Vol. 23, No. 1, 01.02.2014, p. 75-79.

Research output: Contribution to journalArticle

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