Rapid quantification of lipids in acremonium chrysogenum using Oil red O

Hyun Yong Shin, Jin Young Lee, Eun Ji Kim, Seung Wook Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A method based on staining condition and volume of culture broth for the rapid estimation of the level of intracellular lipids in Acremonium chrysogenum using Oil red O was developed. Lipids in A. chrysogenum were strongly stained by the modified Oil red O after treatment for 10 min at 75°C. The results of the study indicated that the Oil red O staining method developed here is useful for the quantification of 0.1-5 mg ml-1 of lipids in A. chrysogenum.

Original languageEnglish
Pages (from-to)1023-1027
Number of pages5
JournalCurrent Microbiology
Volume62
Issue number3
DOIs
Publication statusPublished - 2011 Mar 1

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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