Rapid quantification of lipids in acremonium chrysogenum using Oil red O

Hyun Yong Shin, Jin Young Lee, Eun Ji Kim, Seung Wook Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A method based on staining condition and volume of culture broth for the rapid estimation of the level of intracellular lipids in Acremonium chrysogenum using Oil red O was developed. Lipids in A. chrysogenum were strongly stained by the modified Oil red O after treatment for 10 min at 75°C. The results of the study indicated that the Oil red O staining method developed here is useful for the quantification of 0.1-5 mg ml-1 of lipids in A. chrysogenum.

Original languageEnglish
Pages (from-to)1023-1027
Number of pages5
JournalCurrent Microbiology
Volume62
Issue number3
DOIs
Publication statusPublished - 2011 Mar 1

Fingerprint

Acremonium
Lipids
Staining and Labeling
oil red O

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Rapid quantification of lipids in acremonium chrysogenum using Oil red O. / Shin, Hyun Yong; Lee, Jin Young; Kim, Eun Ji; Kim, Seung Wook.

In: Current Microbiology, Vol. 62, No. 3, 01.03.2011, p. 1023-1027.

Research output: Contribution to journalArticle

Shin, Hyun Yong ; Lee, Jin Young ; Kim, Eun Ji ; Kim, Seung Wook. / Rapid quantification of lipids in acremonium chrysogenum using Oil red O. In: Current Microbiology. 2011 ; Vol. 62, No. 3. pp. 1023-1027.
@article{df1dbebacbb24f6e91f133afdb9dba73,
title = "Rapid quantification of lipids in acremonium chrysogenum using Oil red O",
abstract = "A method based on staining condition and volume of culture broth for the rapid estimation of the level of intracellular lipids in Acremonium chrysogenum using Oil red O was developed. Lipids in A. chrysogenum were strongly stained by the modified Oil red O after treatment for 10 min at 75°C. The results of the study indicated that the Oil red O staining method developed here is useful for the quantification of 0.1-5 mg ml-1 of lipids in A. chrysogenum.",
author = "Shin, {Hyun Yong} and Lee, {Jin Young} and Kim, {Eun Ji} and Kim, {Seung Wook}",
year = "2011",
month = "3",
day = "1",
doi = "10.1007/s00284-010-9818-8",
language = "English",
volume = "62",
pages = "1023--1027",
journal = "Current Microbiology",
issn = "0343-8651",
publisher = "Springer New York",
number = "3",

}

TY - JOUR

T1 - Rapid quantification of lipids in acremonium chrysogenum using Oil red O

AU - Shin, Hyun Yong

AU - Lee, Jin Young

AU - Kim, Eun Ji

AU - Kim, Seung Wook

PY - 2011/3/1

Y1 - 2011/3/1

N2 - A method based on staining condition and volume of culture broth for the rapid estimation of the level of intracellular lipids in Acremonium chrysogenum using Oil red O was developed. Lipids in A. chrysogenum were strongly stained by the modified Oil red O after treatment for 10 min at 75°C. The results of the study indicated that the Oil red O staining method developed here is useful for the quantification of 0.1-5 mg ml-1 of lipids in A. chrysogenum.

AB - A method based on staining condition and volume of culture broth for the rapid estimation of the level of intracellular lipids in Acremonium chrysogenum using Oil red O was developed. Lipids in A. chrysogenum were strongly stained by the modified Oil red O after treatment for 10 min at 75°C. The results of the study indicated that the Oil red O staining method developed here is useful for the quantification of 0.1-5 mg ml-1 of lipids in A. chrysogenum.

UR - http://www.scopus.com/inward/record.url?scp=79954448003&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79954448003&partnerID=8YFLogxK

U2 - 10.1007/s00284-010-9818-8

DO - 10.1007/s00284-010-9818-8

M3 - Article

C2 - 21104083

AN - SCOPUS:79954448003

VL - 62

SP - 1023

EP - 1027

JO - Current Microbiology

JF - Current Microbiology

SN - 0343-8651

IS - 3

ER -