Abstract
The aim of this work was to develop a new approach for simple and high-throughput selection of astaxanthin-hyperproducing Haematococcus mutants through a sequential combination method of azide-based colorimetric assessment and oil-based astaxanthin quantification. Randomly mutagenized cells were spotted on solid culture medium containing 50 µM of sodium azide to accelerate the biosynthesis of astaxanthin. After 3 days, highly-induced mutants were preliminarily isolated by visual inspection and their astaxanthin accumulations were rapidly quantified by soybean oil-based extraction method. On the whole, the selected mutants showed reduced vegetative growth rates but eventually exhibited higher astaxanthin productions than the parental strain owing to their improved inductive growths. Among them, M13 showed 174.7 ± 5.69 mg L−1 of the highest astaxanthin production, which is 1.59-times higher than that of wild-type. This wide-scope screening method expedites both upstream and downstream astaxanthin quantification, making it a useful tool for isolating microalgae with high astaxanthin production.
| Original language | English |
|---|---|
| Pages (from-to) | 175-181 |
| Number of pages | 7 |
| Journal | Bioresource Technology |
| Volume | 267 |
| DOIs | |
| Publication status | Published - 2018 Nov 1 |
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Keywords
- Autotrophic astaxanthin production
- Azide-based colorimetric assay
- Haematococcus pluvialis
- High-throughput screening
- Oil-based astaxanthin extraction method
ASJC Scopus subject areas
- Bioengineering
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal
Cite this
Rapid selection of astaxanthin-hyperproducing Haematococcus mutant via azide-based colorimetric assay combined with oil-based astaxanthin extraction. / Hong, Min Eui; Choi, Hong Il; Kwak, Ho Seok; Hwang, Sung Won; Sung, Young Joon; Chang, Won Seok; Sim, Sang Jun.
In: Bioresource Technology, Vol. 267, 01.11.2018, p. 175-181.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid selection of astaxanthin-hyperproducing Haematococcus mutant via azide-based colorimetric assay combined with oil-based astaxanthin extraction
AU - Hong, Min Eui
AU - Choi, Hong Il
AU - Kwak, Ho Seok
AU - Hwang, Sung Won
AU - Sung, Young Joon
AU - Chang, Won Seok
AU - Sim, Sang Jun
PY - 2018/11/1
Y1 - 2018/11/1
N2 - The aim of this work was to develop a new approach for simple and high-throughput selection of astaxanthin-hyperproducing Haematococcus mutants through a sequential combination method of azide-based colorimetric assessment and oil-based astaxanthin quantification. Randomly mutagenized cells were spotted on solid culture medium containing 50 µM of sodium azide to accelerate the biosynthesis of astaxanthin. After 3 days, highly-induced mutants were preliminarily isolated by visual inspection and their astaxanthin accumulations were rapidly quantified by soybean oil-based extraction method. On the whole, the selected mutants showed reduced vegetative growth rates but eventually exhibited higher astaxanthin productions than the parental strain owing to their improved inductive growths. Among them, M13 showed 174.7 ± 5.69 mg L−1 of the highest astaxanthin production, which is 1.59-times higher than that of wild-type. This wide-scope screening method expedites both upstream and downstream astaxanthin quantification, making it a useful tool for isolating microalgae with high astaxanthin production.
AB - The aim of this work was to develop a new approach for simple and high-throughput selection of astaxanthin-hyperproducing Haematococcus mutants through a sequential combination method of azide-based colorimetric assessment and oil-based astaxanthin quantification. Randomly mutagenized cells were spotted on solid culture medium containing 50 µM of sodium azide to accelerate the biosynthesis of astaxanthin. After 3 days, highly-induced mutants were preliminarily isolated by visual inspection and their astaxanthin accumulations were rapidly quantified by soybean oil-based extraction method. On the whole, the selected mutants showed reduced vegetative growth rates but eventually exhibited higher astaxanthin productions than the parental strain owing to their improved inductive growths. Among them, M13 showed 174.7 ± 5.69 mg L−1 of the highest astaxanthin production, which is 1.59-times higher than that of wild-type. This wide-scope screening method expedites both upstream and downstream astaxanthin quantification, making it a useful tool for isolating microalgae with high astaxanthin production.
KW - Autotrophic astaxanthin production
KW - Azide-based colorimetric assay
KW - Haematococcus pluvialis
KW - High-throughput screening
KW - Oil-based astaxanthin extraction method
UR - http://www.scopus.com/inward/record.url?scp=85049784692&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85049784692&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2018.07.033
DO - 10.1016/j.biortech.2018.07.033
M3 - Article
C2 - 30014996
AN - SCOPUS:85049784692
VL - 267
SP - 175
EP - 181
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -