Rapid selection of astaxanthin-hyperproducing Haematococcus mutant via azide-based colorimetric assay combined with oil-based astaxanthin extraction

Min Eui Hong, Hong Il Choi, Ho Seok Kwak, Sung Won Hwang, Young Joon Sung, Won Seok Chang, Sang Jun Sim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The aim of this work was to develop a new approach for simple and high-throughput selection of astaxanthin-hyperproducing Haematococcus mutants through a sequential combination method of azide-based colorimetric assessment and oil-based astaxanthin quantification. Randomly mutagenized cells were spotted on solid culture medium containing 50 µM of sodium azide to accelerate the biosynthesis of astaxanthin. After 3 days, highly-induced mutants were preliminarily isolated by visual inspection and their astaxanthin accumulations were rapidly quantified by soybean oil-based extraction method. On the whole, the selected mutants showed reduced vegetative growth rates but eventually exhibited higher astaxanthin productions than the parental strain owing to their improved inductive growths. Among them, M13 showed 174.7 ± 5.69 mg L−1 of the highest astaxanthin production, which is 1.59-times higher than that of wild-type. This wide-scope screening method expedites both upstream and downstream astaxanthin quantification, making it a useful tool for isolating microalgae with high astaxanthin production.

Original languageEnglish
Pages (from-to)175-181
Number of pages7
JournalBioresource Technology
Volume267
DOIs
Publication statusPublished - 2018 Nov 1

Fingerprint

Azides
Assays
Oils
assay
oil
Soybean oil
Biosynthesis
extraction method
soybean
Screening
Inspection
Sodium
Throughput
sodium
Sodium Azide
Soybean Oil
astaxanthine
mutant
Culture Media
method

Keywords

  • Autotrophic astaxanthin production
  • Azide-based colorimetric assay
  • Haematococcus pluvialis
  • High-throughput screening
  • Oil-based astaxanthin extraction method

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Rapid selection of astaxanthin-hyperproducing Haematococcus mutant via azide-based colorimetric assay combined with oil-based astaxanthin extraction. / Hong, Min Eui; Choi, Hong Il; Kwak, Ho Seok; Hwang, Sung Won; Sung, Young Joon; Chang, Won Seok; Sim, Sang Jun.

In: Bioresource Technology, Vol. 267, 01.11.2018, p. 175-181.

Research output: Contribution to journalArticle

Hong, Min Eui ; Choi, Hong Il ; Kwak, Ho Seok ; Hwang, Sung Won ; Sung, Young Joon ; Chang, Won Seok ; Sim, Sang Jun. / Rapid selection of astaxanthin-hyperproducing Haematococcus mutant via azide-based colorimetric assay combined with oil-based astaxanthin extraction. In: Bioresource Technology. 2018 ; Vol. 267. pp. 175-181.
@article{c531574027374c30b169f5a88d500717,
title = "Rapid selection of astaxanthin-hyperproducing Haematococcus mutant via azide-based colorimetric assay combined with oil-based astaxanthin extraction",
abstract = "The aim of this work was to develop a new approach for simple and high-throughput selection of astaxanthin-hyperproducing Haematococcus mutants through a sequential combination method of azide-based colorimetric assessment and oil-based astaxanthin quantification. Randomly mutagenized cells were spotted on solid culture medium containing 50 µM of sodium azide to accelerate the biosynthesis of astaxanthin. After 3 days, highly-induced mutants were preliminarily isolated by visual inspection and their astaxanthin accumulations were rapidly quantified by soybean oil-based extraction method. On the whole, the selected mutants showed reduced vegetative growth rates but eventually exhibited higher astaxanthin productions than the parental strain owing to their improved inductive growths. Among them, M13 showed 174.7 ± 5.69 mg L−1 of the highest astaxanthin production, which is 1.59-times higher than that of wild-type. This wide-scope screening method expedites both upstream and downstream astaxanthin quantification, making it a useful tool for isolating microalgae with high astaxanthin production.",
keywords = "Autotrophic astaxanthin production, Azide-based colorimetric assay, Haematococcus pluvialis, High-throughput screening, Oil-based astaxanthin extraction method",
author = "Hong, {Min Eui} and Choi, {Hong Il} and Kwak, {Ho Seok} and Hwang, {Sung Won} and Sung, {Young Joon} and Chang, {Won Seok} and Sim, {Sang Jun}",
year = "2018",
month = "11",
day = "1",
doi = "10.1016/j.biortech.2018.07.033",
language = "English",
volume = "267",
pages = "175--181",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Rapid selection of astaxanthin-hyperproducing Haematococcus mutant via azide-based colorimetric assay combined with oil-based astaxanthin extraction

AU - Hong, Min Eui

AU - Choi, Hong Il

AU - Kwak, Ho Seok

AU - Hwang, Sung Won

AU - Sung, Young Joon

AU - Chang, Won Seok

AU - Sim, Sang Jun

PY - 2018/11/1

Y1 - 2018/11/1

N2 - The aim of this work was to develop a new approach for simple and high-throughput selection of astaxanthin-hyperproducing Haematococcus mutants through a sequential combination method of azide-based colorimetric assessment and oil-based astaxanthin quantification. Randomly mutagenized cells were spotted on solid culture medium containing 50 µM of sodium azide to accelerate the biosynthesis of astaxanthin. After 3 days, highly-induced mutants were preliminarily isolated by visual inspection and their astaxanthin accumulations were rapidly quantified by soybean oil-based extraction method. On the whole, the selected mutants showed reduced vegetative growth rates but eventually exhibited higher astaxanthin productions than the parental strain owing to their improved inductive growths. Among them, M13 showed 174.7 ± 5.69 mg L−1 of the highest astaxanthin production, which is 1.59-times higher than that of wild-type. This wide-scope screening method expedites both upstream and downstream astaxanthin quantification, making it a useful tool for isolating microalgae with high astaxanthin production.

AB - The aim of this work was to develop a new approach for simple and high-throughput selection of astaxanthin-hyperproducing Haematococcus mutants through a sequential combination method of azide-based colorimetric assessment and oil-based astaxanthin quantification. Randomly mutagenized cells were spotted on solid culture medium containing 50 µM of sodium azide to accelerate the biosynthesis of astaxanthin. After 3 days, highly-induced mutants were preliminarily isolated by visual inspection and their astaxanthin accumulations were rapidly quantified by soybean oil-based extraction method. On the whole, the selected mutants showed reduced vegetative growth rates but eventually exhibited higher astaxanthin productions than the parental strain owing to their improved inductive growths. Among them, M13 showed 174.7 ± 5.69 mg L−1 of the highest astaxanthin production, which is 1.59-times higher than that of wild-type. This wide-scope screening method expedites both upstream and downstream astaxanthin quantification, making it a useful tool for isolating microalgae with high astaxanthin production.

KW - Autotrophic astaxanthin production

KW - Azide-based colorimetric assay

KW - Haematococcus pluvialis

KW - High-throughput screening

KW - Oil-based astaxanthin extraction method

UR - http://www.scopus.com/inward/record.url?scp=85049784692&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049784692&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2018.07.033

DO - 10.1016/j.biortech.2018.07.033

M3 - Article

VL - 267

SP - 175

EP - 181

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -