RASSF1A suppresses oncogenic H-Ras-induced c-Jun N-terminal kinase activation

Young A. Yoo, Ah Ram Na, Myeong Sok Lee, Sukjoon Yoon, Jun Suk Kim, Young Do Yoo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The constitutive activation of JNK has been implicated in Ras-induced cellular transformation and activated JNK is down-regulated by the tumor suppressor protein, RASSF1A. In this study, we examined whether RASSF1A blocked oncogenic Ras-induced JNK activation. Exogenous expression of H-Ras G12V induced JNK phosphorylation and RASSF1A co-transfected with H-RasG12V efficiently suppressed Ras-triggered JNK activation in various cancer cell lines. RASSF1A expression revived the H-Ras G12V-induced p27Kip1 down-regulation. JNK siRNA treatment also promoted recovery from the H-RasG12V-induced p27Kip1 downregulation. These results demonstrate that RASSF1A inhibited H-Ras G12V-induced JNK activation and JNK-mediated p27Kip1 down-regulation. From these results, we propose that RASSF1A exerts a tumor-suppressing effect by blocking oncogenic Ras-induced JNK activation.

Original languageEnglish
Pages (from-to)1541-1547
Number of pages7
JournalInternational journal of oncology
Volume29
Issue number6
Publication statusPublished - 2006 Dec 1

Keywords

  • H-Ras
  • JNK
  • RASSF1A
  • p21
  • p27

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Yoo, Y. A., Na, A. R., Lee, M. S., Yoon, S., Kim, J. S., & Yoo, Y. D. (2006). RASSF1A suppresses oncogenic H-Ras-induced c-Jun N-terminal kinase activation. International journal of oncology, 29(6), 1541-1547.