RASSF1A suppresses the c-Jun-NH 2-kinase pathway and inhibits cell cycle progression

Young Mi Whang, Yeul Hong Kim, Jun Suk Kim, Young Do Yoo

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Some oncogenes, such as activated Ras, cause the malignant transformation of lung cells. c-Jun-NH 2-kinase (JNK) activation is essential for the oncogenic function of these cells. In this study, we show that RASSF1A inhibits the growth of lung cancer cells by blocking the JNK pathway. The exogenous expression of RASSF1A suppressed JNK phosphorylation, and cells stably transfected with RASSF1A showed reduced JNK and c-Jun phosphorylation and Cyclin D1 down-regulation. An in vitro kinase assay showed that the exogenous expression of RASSF1A inhibited JNK activity and that JNK activity suppression due to ectopically expressed RASSF1A was revived by RASSF1A siRNA treatment. Based on our data, we suggest that RASSF1A exerts a tumor-suppressing effect by blocking oncogene-mediated JNK activation in lung cells.

Original languageEnglish
Pages (from-to)3682-3690
Number of pages9
JournalCancer Research
Volume65
Issue number9
DOIs
Publication statusPublished - 2005 May 1

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Mitogen-Activated Protein Kinase 9
Cell Cycle
Oncogenes
Phosphorylation
Lung
Cyclin D1
Small Interfering RNA
Lung Neoplasms
Phosphotransferases
Down-Regulation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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RASSF1A suppresses the c-Jun-NH 2-kinase pathway and inhibits cell cycle progression. / Whang, Young Mi; Kim, Yeul Hong; Kim, Jun Suk; Yoo, Young Do.

In: Cancer Research, Vol. 65, No. 9, 01.05.2005, p. 3682-3690.

Research output: Contribution to journalArticle

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