Ratiometric detection of mitochondrial thiols with a two-photon fluorescent probe

Chang Su Lim, Goutam Masanta, Hyung Joong Kim, Ji Hee Han, Hwan Myung Kim, Bong Rae Cho

Research output: Contribution to journalArticlepeer-review

320 Citations (Scopus)

Abstract

We report a ratiometric two-photon probe (SSH-Mito) for mitochondrial thiols. This probe shows a marked blue-to-yellow emission color change in response to RSH, a significant two-photon cross section, good mitochondrial thiol selectivity, low cytotoxicity, and insensitivity to pH over the biologically relevant pH range, allowing the direct visualization of RSH levels in live cells as well as in living tissues at 90 - 190 |m depth without interference from other biologically relevant species through the use of two-photon microscopy.

Original languageEnglish
Pages (from-to)11132-11135
Number of pages4
JournalJournal of the American Chemical Society
Volume133
Issue number29
DOIs
Publication statusPublished - 2011 Jul 27

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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