TY - JOUR
T1 - Ratiometric fluorescent probe for monitoring tyrosinase activity in melanosomes of melanoma cancer cells
AU - Park, Sun Young
AU - Won, Miae
AU - Kim, Jong Seung
AU - Lee, Min Hee
N1 - Funding Information:
This research was supported by the CRI project (2018R1A3B1052702, J.S.K.) and National Research Foundation of Korea (NRF) (2017R1D1A1B03030062, M.W.; 2018R1C1B6006110, M.H.L.).
Funding Information:
This research was supported by the CRI project ( 2018R1A3B1052702 , J.S.K.) and National Research Foundation of Korea (NRF) ( 2017R1D1A1B03030062 , M.W.; 2018R1C1B6006110 , M.H.L.).
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/9/15
Y1 - 2020/9/15
N2 - Tyrosinase is an important biomarker of melanoma cancer. We herein report a melanosome targetable naphthalimide (1) as a ratiometric fluorescent probe that allows for a more precise, quantitative, and spatio-temporal analyses of tyrosinase activity. This probe can provide a ratiometric response to the melanosomal tyrosinase. By taking advantage of ratiometric response, probe 1 can be used for high-throughput screening that accurately addresses the efficacy of therapeutic agents related to tyrosinase activity. Moreover, real-time monitoring of melanosomal tyrosinase degradation by a melanogenesis inhibitor in melanoma cancer cells can be precisely achieved by dual channel confocal microscopic imaging.
AB - Tyrosinase is an important biomarker of melanoma cancer. We herein report a melanosome targetable naphthalimide (1) as a ratiometric fluorescent probe that allows for a more precise, quantitative, and spatio-temporal analyses of tyrosinase activity. This probe can provide a ratiometric response to the melanosomal tyrosinase. By taking advantage of ratiometric response, probe 1 can be used for high-throughput screening that accurately addresses the efficacy of therapeutic agents related to tyrosinase activity. Moreover, real-time monitoring of melanosomal tyrosinase degradation by a melanogenesis inhibitor in melanoma cancer cells can be precisely achieved by dual channel confocal microscopic imaging.
KW - Melanogenesis inhibitor
KW - Melanoma cancer
KW - Melanosomal tyrosinase
KW - Ratiometric fluorescent probe
KW - Tyrosinase detection
UR - http://www.scopus.com/inward/record.url?scp=85085514119&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2020.128306
DO - 10.1016/j.snb.2020.128306
M3 - Article
AN - SCOPUS:85085514119
SN - 0925-4005
VL - 319
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
M1 - 128306
ER -
