Ratiometric fluorescent probe for monitoring tyrosinase activity in melanosomes of melanoma cancer cells

Sun Young Park, Miae Won, Jong Seung Kim, Min Hee Lee

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Tyrosinase is an important biomarker of melanoma cancer. We herein report a melanosome targetable naphthalimide (1) as a ratiometric fluorescent probe that allows for a more precise, quantitative, and spatio-temporal analyses of tyrosinase activity. This probe can provide a ratiometric response to the melanosomal tyrosinase. By taking advantage of ratiometric response, probe 1 can be used for high-throughput screening that accurately addresses the efficacy of therapeutic agents related to tyrosinase activity. Moreover, real-time monitoring of melanosomal tyrosinase degradation by a melanogenesis inhibitor in melanoma cancer cells can be precisely achieved by dual channel confocal microscopic imaging.

Original languageEnglish
Article number128306
JournalSensors and Actuators, B: Chemical
Volume319
DOIs
Publication statusPublished - 2020 Sep 15

Keywords

  • Melanogenesis inhibitor
  • Melanoma cancer
  • Melanosomal tyrosinase
  • Ratiometric fluorescent probe
  • Tyrosinase detection

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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