Rational design of a photo-crosslinking BODIPY for in situ protein labeling

Dhiraj P. Murale, Seong Cheol Hong, Ji Hyeon Yun, Chang No Yoon, Jun Seok Lee

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Photo-crosslinking agents have emerged as critical tools to investigate protein-protein interactions in complex proteomes, but there are few photocrosslinkers available at the moment. Here, we report the first rational design of a photo-crosslinking BODIPY fluorophore (pcBD) and its biological application for biomolecule labeling. As a photosensitizing functional motif, an aryl ketone group was incorporated into the BODIPY fluorophore, and a series of proteins were labeled by pcBD compounds upon UV irradiation. In order to investigate protein-protein interactions in a protein mixture, amino-functionalized pcBD was prepared and covalently attached to a ubiquitin ligase binding peptide. Upon UV irradiation, we could successfully visualize the substrates in the total lysate. These results provided a proof of concept for spatially controllable tagging via photo-activation of the pcBD scaffold, and demonstrated its potential usage for in situ labeling applications.

Original languageEnglish
Pages (from-to)6643-6646
Number of pages4
JournalChemical Communications
Volume51
Issue number30
DOIs
Publication statusPublished - 2015 Apr 18
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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