Rck1 promotes pseudohyphal growth via the activation of Ubp3 phosphorylation in Saccharomyces cerevisiae

Chang Min Kang, Miwha Chang, Yong Sung Park, Cheol-Won Yun

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Previously, we reported that Rck1 up-regulates Ras2 and pseudohyphal growth of Saccharomyces cerevisiae. Here, we further investigate the involvement of Rck1 in the activation of pseudohyphal growth. Rck1 activated phosphorylation of the deubiquitinase Ubp3 through a direct protein interaction between Rck1 and Ubp3. The N-terminal Bre5 binding region of Ubp3 physically interacted with Rck1, and Ubp3 and Rck1 co-precipitated. Overexpression of UBP3 using a high-copy plasmid resulted in the upregulation of Ras2, and deletion of UBP3 blocked the upregulation of Ras2 by RCK1 overexpression. Treatment with the proteasome inhibitor MG132 resulted in accumulation of Ras2, indicating that Rck1 is involved in Ras2 degradation in a proteasome-dependent manner. Furthermore, deletion of UBP3 blocked the upregulation of FLO11, a flocculin required for pseudohyphal and invasive growth induced by RCK1 overexpression in S. cerevisiae. Taken together, these results demonstrate that Rck1 promotes S. cerevisiae pseudohyphal growth via the activation of Ubp3 phosphorylation.

Original languageEnglish
Pages (from-to)333-339
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume469
Issue number3
DOIs
Publication statusPublished - 2016 Jan 15

Fingerprint

Phosphorylation
Yeast
Saccharomyces cerevisiae
Up-Regulation
Chemical activation
Growth
Proteasome Inhibitors
Proteasome Endopeptidase Complex
Plasmids
Degradation
Proteins

Keywords

  • Pseudohyphal growth
  • Ras2
  • Rck1
  • S. cerevisiae
  • Ubp3

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Rck1 promotes pseudohyphal growth via the activation of Ubp3 phosphorylation in Saccharomyces cerevisiae. / Kang, Chang Min; Chang, Miwha; Park, Yong Sung; Yun, Cheol-Won.

In: Biochemical and Biophysical Research Communications, Vol. 469, No. 3, 15.01.2016, p. 333-339.

Research output: Contribution to journalArticle

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