Rck1 up-regulates Hog1 activity by down-regulating Slt2 activity in Saccharomyces cerevisiae

Miwha Chang, Hyun Jun Kang, In Joon Baek, Chang Min Kang, Yong Sung Park, Cheol-Won Yun

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We previously reported that the over-expression of KDX1 up-regulates RCK1 gene expression. To further understand the function of Rck1, microarray analysis was performed using a RCK1 over-expressing strain. Based on microarray and Northern blot analyses, we determined that the expression of KDX1 was down-regulated when RCK1 was over-expressed. Furthermore, we determined that phosphorylated forms of Slt2 and Mkk2 were down-regulated by the over-expression of RCK1. Ptp2, a phosphatase that is regulated by the Slt2 MAP kinase pathway, was down-regulated by the over-expression of RCK1. Ptp2 is a negative regulator of Hog1; thus, the phosphorylated form of Hog1 was up-regulated by RCK1 over-expression. A point mutation of lysine 152 to arginine resulted in a failure to up-regulate Hog1 and the subsequent down-regulation of CTT1, which is a Hog1 pathway target gene. Furthermore, using microarray and Northern blot analyses, we determined that genes that are regulated by Msn2/Msn4 were up-regulated by Rck1 and that this was the result of Hog1 activation by RCK1 over-expression. Together, our results suggest that Rck1 inhibits Slt2 MAP kinase pathway activity and then Ptp2, which subsequently activates Hog1.

Original languageEnglish
Pages (from-to)119-124
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume440
Issue number1
DOIs
Publication statusPublished - 2013 Oct 11

Fingerprint

Microarrays
Northern Blotting
Yeast
Saccharomyces cerevisiae
Phosphotransferases
Up-Regulation
Microarray Analysis
Genes
Phosphoric Monoester Hydrolases
Point Mutation
Lysine
Arginine
Down-Regulation
Gene Expression
Gene expression
Chemical activation

Keywords

  • Kdx1
  • Mkk2
  • Rck1
  • Saccharomyces cerevisiae
  • Slt2

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Rck1 up-regulates Hog1 activity by down-regulating Slt2 activity in Saccharomyces cerevisiae. / Chang, Miwha; Kang, Hyun Jun; Baek, In Joon; Kang, Chang Min; Park, Yong Sung; Yun, Cheol-Won.

In: Biochemical and Biophysical Research Communications, Vol. 440, No. 1, 11.10.2013, p. 119-124.

Research output: Contribution to journalArticle

Chang, Miwha ; Kang, Hyun Jun ; Baek, In Joon ; Kang, Chang Min ; Park, Yong Sung ; Yun, Cheol-Won. / Rck1 up-regulates Hog1 activity by down-regulating Slt2 activity in Saccharomyces cerevisiae. In: Biochemical and Biophysical Research Communications. 2013 ; Vol. 440, No. 1. pp. 119-124.
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