Rck1 up-regulates pseudohyphal growth by activating the Ras2 and MAP kinase pathways independently in Saccharomyces cerevisiae

Miwha Chang, Chang Min Kang, Yong Sung Park, Cheol-Won Yun

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Previously, we reported that Rck1 regulates Hog1 and Slt2 activities and affects MAP kinase activity in Saccharomyces cerevisiae. Recently, we found that Rck1 up-regulates phospho-Kss1 and phospho-Fus3. Kss1 has been known as a component in the pseudohyphal growth pathway, and we attempted to identify the function of Rck1 in pseudohyphal growth. Rck1 up-regulated Ras2 at the protein level, not the transcriptional level. Additionally, FLO11 transcription was up-regulated by RCK1 over-expression. RCK1 expression was up-regulated during growth on SLAD + 1% butanol medium. On nitrogen starvation agar plates, RCK1 over-expression induced pseudohyphal growth of colonies, and cells over-expressing RCK1 showed a filamentous morphology when grown in SLAD medium. Furthermore, 1-butanol greatly induced filamentous growth when RCK1 was over-expressed. Moreover, invasive growth was activated in haploid cells when RCK1 was over-expressed. The growth defect of cells observed on 1-butanol medium was recovered when RCK1 was over-expressed. Interestingly, Ras2 and phospho-Kss1 were up-regulated by Rck1 independently. Together, these results suggest that Rck1 promotes pseudohyphal growth by activating Ras2 and Kss1 via independent pathways in S. Cerevisiae.

Original languageEnglish
Pages (from-to)656-661
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume444
Issue number4
DOIs
Publication statusPublished - 2014 Feb 21

Fingerprint

Yeast
Saccharomyces cerevisiae
Phosphotransferases
Up-Regulation
Growth
1-Butanol
Haploidy
Transcription
Starvation
Agar
Nitrogen
Defects
Proteins

Keywords

  • Kss1
  • MAP kinase
  • Ras2
  • Rck1
  • S. Cerevisiae

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Rck1 up-regulates pseudohyphal growth by activating the Ras2 and MAP kinase pathways independently in Saccharomyces cerevisiae. / Chang, Miwha; Kang, Chang Min; Park, Yong Sung; Yun, Cheol-Won.

In: Biochemical and Biophysical Research Communications, Vol. 444, No. 4, 21.02.2014, p. 656-661.

Research output: Contribution to journalArticle

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