TY - JOUR
T1 - Re-consideration of Peronospora farinosa infecting Spinacia oleracea as distinct species, Peronospora effusa
AU - Choi, Young Joon
AU - Hong, Seung Beom
AU - Shin, Hyeon Dong
N1 - Funding Information:
The authors are grateful to Ovidiu Constantinescu (Uppsala University), for his helpful comments and suggestions, to the curators of BPI, DM, E, HMAS, LE, BRNM, PDD, SOMF, UPS, and VPRI for providing the Peronospora specimens, and to the staff at the Korean Agricultural Culture Collection for their technical assistance in the DNA sequencing and phylogenetic analyses. This work was financially supported by a research grant from the Korea Research Foundation (KRF-2004-015-C00508).
PY - 2007/4
Y1 - 2007/4
N2 - Downy mildew is probably the most widespread and potentially destructive global disease of spinach (Spinacia oleracea). The causal agent of downy mildew disease on various plants of Chenopodiaceae, including spinach, is regarded as a single species, Peronospora farinosa. In the present study, the ITS rDNA sequence and morphological data demonstrated that P. farinosa from S. oleracea is distinct from downy mildew of other chenopodiaceous hosts. Fifty-eight spinach specimens were collected or loaned from 17 countries of Asia, Europe, Oceania, North and South America, which all formed a distinct monophyletic group. No intercontinental genetic variation of the ITS rDNA within Peronospora accessions causing spinach downy mildew disease was found. Phylogenetic trees supported recognition of Peronospora from spinach as a separate species. Microscopic examination also revealed morphological differences between Peronospora specimens from Spinacia and P. farinosa s. lat. specimens from Atriplex, Bassia, Beta, and Chenopodium. Consequently, the name Peronospora effusa should be reinstated for the downy mildew fungus found on spinach. Here, a specimen of the original collections of Peronospora effusa is designated as lectotype.
AB - Downy mildew is probably the most widespread and potentially destructive global disease of spinach (Spinacia oleracea). The causal agent of downy mildew disease on various plants of Chenopodiaceae, including spinach, is regarded as a single species, Peronospora farinosa. In the present study, the ITS rDNA sequence and morphological data demonstrated that P. farinosa from S. oleracea is distinct from downy mildew of other chenopodiaceous hosts. Fifty-eight spinach specimens were collected or loaned from 17 countries of Asia, Europe, Oceania, North and South America, which all formed a distinct monophyletic group. No intercontinental genetic variation of the ITS rDNA within Peronospora accessions causing spinach downy mildew disease was found. Phylogenetic trees supported recognition of Peronospora from spinach as a separate species. Microscopic examination also revealed morphological differences between Peronospora specimens from Spinacia and P. farinosa s. lat. specimens from Atriplex, Bassia, Beta, and Chenopodium. Consequently, the name Peronospora effusa should be reinstated for the downy mildew fungus found on spinach. Here, a specimen of the original collections of Peronospora effusa is designated as lectotype.
KW - Chenopodiaceae
KW - Downy mildew
KW - ITS rDNA
KW - Phylogenetic analysis
KW - Spinach
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U2 - 10.1016/j.mycres.2007.02.003
DO - 10.1016/j.mycres.2007.02.003
M3 - Article
C2 - 17509849
AN - SCOPUS:34249287352
VL - 111
SP - 381
EP - 391
JO - Fungal Biology
JF - Fungal Biology
SN - 1878-6146
IS - 4
ER -