Abstract
Bacillus strain JF-2 (ATCC 39307) is a halotolerant, biosurfactant-producing bacterium that was initially described as a member of the species Bacillus licheniformis based on a limited set of phenotypic characteristics. Here, genetic and phenotypic analyses were employed to determine the relationship of Bacillus strain JF-2 to other Bacillus strains. The restriction patterns with AluI and analysis of gyrA and 16S rRNA gene sequences grouped Bacillus strain JF-2 with B. mojavensisT and not with B. licheniformisT. DNA-DNA similarity showed JF-2 was 75% similar to B. mojavensisT and only 11% similar to B. licheniformisT. Both strain JF-2 and B. mojavensisT required DNA for anaerobic growth, but B. licheniformisT did not. B. mojavensisT and strain JF-2 did not grow anaerobically in thioglycollate medium or aerobically with propionate while B. licheniformisT grew under these conditions. DNA-DNA similarity, gene sequence data and phenotypic characteristics all support the assignment of JF-2 as a member of the species B. mojavensis.
| Original language | English |
|---|---|
| Pages (from-to) | 645-649 |
| Number of pages | 5 |
| Journal | Systematic and Applied Microbiology |
| Volume | 29 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2006 Dec 4 |
| Externally published | Yes |
Fingerprint
Keywords
- 16S rRNA gene
- Anaerobic growth
- Bacillus licheniformis
- Bacillus mojavensis
- Bacillus subtilis
- DNA-DNA similarity
- Phylogeny
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
Cite this
Re-identification of the halotolerant, biosurfactant-producing Bacillus licheniformis strain JF-2 as Bacillus mojavensis strain JF-2. / Folmsbee, Martha; Duncan, Kathleen; Han, Sung Ok; Nagle, David; Jennings, Eleanor; McInerney, Michael.
In: Systematic and Applied Microbiology, Vol. 29, No. 8, 04.12.2006, p. 645-649.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Re-identification of the halotolerant, biosurfactant-producing Bacillus licheniformis strain JF-2 as Bacillus mojavensis strain JF-2
AU - Folmsbee, Martha
AU - Duncan, Kathleen
AU - Han, Sung Ok
AU - Nagle, David
AU - Jennings, Eleanor
AU - McInerney, Michael
PY - 2006/12/4
Y1 - 2006/12/4
N2 - Bacillus strain JF-2 (ATCC 39307) is a halotolerant, biosurfactant-producing bacterium that was initially described as a member of the species Bacillus licheniformis based on a limited set of phenotypic characteristics. Here, genetic and phenotypic analyses were employed to determine the relationship of Bacillus strain JF-2 to other Bacillus strains. The restriction patterns with AluI and analysis of gyrA and 16S rRNA gene sequences grouped Bacillus strain JF-2 with B. mojavensisT and not with B. licheniformisT. DNA-DNA similarity showed JF-2 was 75% similar to B. mojavensisT and only 11% similar to B. licheniformisT. Both strain JF-2 and B. mojavensisT required DNA for anaerobic growth, but B. licheniformisT did not. B. mojavensisT and strain JF-2 did not grow anaerobically in thioglycollate medium or aerobically with propionate while B. licheniformisT grew under these conditions. DNA-DNA similarity, gene sequence data and phenotypic characteristics all support the assignment of JF-2 as a member of the species B. mojavensis.
AB - Bacillus strain JF-2 (ATCC 39307) is a halotolerant, biosurfactant-producing bacterium that was initially described as a member of the species Bacillus licheniformis based on a limited set of phenotypic characteristics. Here, genetic and phenotypic analyses were employed to determine the relationship of Bacillus strain JF-2 to other Bacillus strains. The restriction patterns with AluI and analysis of gyrA and 16S rRNA gene sequences grouped Bacillus strain JF-2 with B. mojavensisT and not with B. licheniformisT. DNA-DNA similarity showed JF-2 was 75% similar to B. mojavensisT and only 11% similar to B. licheniformisT. Both strain JF-2 and B. mojavensisT required DNA for anaerobic growth, but B. licheniformisT did not. B. mojavensisT and strain JF-2 did not grow anaerobically in thioglycollate medium or aerobically with propionate while B. licheniformisT grew under these conditions. DNA-DNA similarity, gene sequence data and phenotypic characteristics all support the assignment of JF-2 as a member of the species B. mojavensis.
KW - 16S rRNA gene
KW - Anaerobic growth
KW - Bacillus licheniformis
KW - Bacillus mojavensis
KW - Bacillus subtilis
KW - DNA-DNA similarity
KW - Phylogeny
UR - http://www.scopus.com/inward/record.url?scp=33751235605&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33751235605&partnerID=8YFLogxK
U2 - 10.1016/j.syapm.2006.01.010
DO - 10.1016/j.syapm.2006.01.010
M3 - Article
VL - 29
SP - 645
EP - 649
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
SN - 0723-2020
IS - 8
ER -