Re-identification of the halotolerant, biosurfactant-producing Bacillus licheniformis strain JF-2 as Bacillus mojavensis strain JF-2

Martha Folmsbee; Kathleen Duncan; Sung Ok Han; David Nagle; Eleanor Jennings; Michael McInerney

doi:10.1016/j.syapm.2006.01.010

Re-identification of the halotolerant, biosurfactant-producing Bacillus licheniformis strain JF-2 as Bacillus mojavensis strain JF-2

Martha Folmsbee, Kathleen Duncan, Sung Ok Han, David Nagle, Eleanor Jennings, Michael McInerney

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Bacillus strain JF-2 (ATCC 39307) is a halotolerant, biosurfactant-producing bacterium that was initially described as a member of the species Bacillus licheniformis based on a limited set of phenotypic characteristics. Here, genetic and phenotypic analyses were employed to determine the relationship of Bacillus strain JF-2 to other Bacillus strains. The restriction patterns with AluI and analysis of gyrA and 16S rRNA gene sequences grouped Bacillus strain JF-2 with B. mojavensis^T and not with B. licheniformis^T. DNA-DNA similarity showed JF-2 was 75% similar to B. mojavensis^T and only 11% similar to B. licheniformis^T. Both strain JF-2 and B. mojavensis^T required DNA for anaerobic growth, but B. licheniformis^T did not. B. mojavensis^T and strain JF-2 did not grow anaerobically in thioglycollate medium or aerobically with propionate while B. licheniformis^T grew under these conditions. DNA-DNA similarity, gene sequence data and phenotypic characteristics all support the assignment of JF-2 as a member of the species B. mojavensis.

Original language	English
Pages (from-to)	645-649
Number of pages	5
Journal	Systematic and Applied Microbiology
Volume	29
Issue number	8
DOIs	https://doi.org/10.1016/j.syapm.2006.01.010
Publication status	Published - 2006 Dec 4
Externally published	Yes

Keywords

16S rRNA gene
Anaerobic growth
Bacillus licheniformis
Bacillus mojavensis
Bacillus subtilis
DNA-DNA similarity
Phylogeny

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics
Applied Microbiology and Biotechnology

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title = "Re-identification of the halotolerant, biosurfactant-producing Bacillus licheniformis strain JF-2 as Bacillus mojavensis strain JF-2",

abstract = "Bacillus strain JF-2 (ATCC 39307) is a halotolerant, biosurfactant-producing bacterium that was initially described as a member of the species Bacillus licheniformis based on a limited set of phenotypic characteristics. Here, genetic and phenotypic analyses were employed to determine the relationship of Bacillus strain JF-2 to other Bacillus strains. The restriction patterns with AluI and analysis of gyrA and 16S rRNA gene sequences grouped Bacillus strain JF-2 with B. mojavensisT and not with B. licheniformisT. DNA-DNA similarity showed JF-2 was 75% similar to B. mojavensisT and only 11% similar to B. licheniformisT. Both strain JF-2 and B. mojavensisT required DNA for anaerobic growth, but B. licheniformisT did not. B. mojavensisT and strain JF-2 did not grow anaerobically in thioglycollate medium or aerobically with propionate while B. licheniformisT grew under these conditions. DNA-DNA similarity, gene sequence data and phenotypic characteristics all support the assignment of JF-2 as a member of the species B. mojavensis.",

keywords = "16S rRNA gene, Anaerobic growth, Bacillus licheniformis, Bacillus mojavensis, Bacillus subtilis, DNA-DNA similarity, Phylogeny",

author = "Martha Folmsbee and Kathleen Duncan and Han, {Sung Ok} and David Nagle and Eleanor Jennings and Michael McInerney",

note = "Funding Information: This work was supported by Department of Energy Contracts DE-FC26-04NT15522 and DE-FG02-96ER20214.",

year = "2006",

month = dec,

day = "4",

doi = "10.1016/j.syapm.2006.01.010",

language = "English",

volume = "29",

pages = "645--649",

journal = "Systematic and Applied Microbiology",

issn = "0723-2020",

publisher = "Urban und Fischer Verlag Jena",

number = "8",

}

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T1 - Re-identification of the halotolerant, biosurfactant-producing Bacillus licheniformis strain JF-2 as Bacillus mojavensis strain JF-2

AU - Folmsbee, Martha

AU - Duncan, Kathleen

AU - Han, Sung Ok

AU - Nagle, David

AU - Jennings, Eleanor

AU - McInerney, Michael

N1 - Funding Information: This work was supported by Department of Energy Contracts DE-FC26-04NT15522 and DE-FG02-96ER20214.

PY - 2006/12/4

Y1 - 2006/12/4

N2 - Bacillus strain JF-2 (ATCC 39307) is a halotolerant, biosurfactant-producing bacterium that was initially described as a member of the species Bacillus licheniformis based on a limited set of phenotypic characteristics. Here, genetic and phenotypic analyses were employed to determine the relationship of Bacillus strain JF-2 to other Bacillus strains. The restriction patterns with AluI and analysis of gyrA and 16S rRNA gene sequences grouped Bacillus strain JF-2 with B. mojavensisT and not with B. licheniformisT. DNA-DNA similarity showed JF-2 was 75% similar to B. mojavensisT and only 11% similar to B. licheniformisT. Both strain JF-2 and B. mojavensisT required DNA for anaerobic growth, but B. licheniformisT did not. B. mojavensisT and strain JF-2 did not grow anaerobically in thioglycollate medium or aerobically with propionate while B. licheniformisT grew under these conditions. DNA-DNA similarity, gene sequence data and phenotypic characteristics all support the assignment of JF-2 as a member of the species B. mojavensis.

AB - Bacillus strain JF-2 (ATCC 39307) is a halotolerant, biosurfactant-producing bacterium that was initially described as a member of the species Bacillus licheniformis based on a limited set of phenotypic characteristics. Here, genetic and phenotypic analyses were employed to determine the relationship of Bacillus strain JF-2 to other Bacillus strains. The restriction patterns with AluI and analysis of gyrA and 16S rRNA gene sequences grouped Bacillus strain JF-2 with B. mojavensisT and not with B. licheniformisT. DNA-DNA similarity showed JF-2 was 75% similar to B. mojavensisT and only 11% similar to B. licheniformisT. Both strain JF-2 and B. mojavensisT required DNA for anaerobic growth, but B. licheniformisT did not. B. mojavensisT and strain JF-2 did not grow anaerobically in thioglycollate medium or aerobically with propionate while B. licheniformisT grew under these conditions. DNA-DNA similarity, gene sequence data and phenotypic characteristics all support the assignment of JF-2 as a member of the species B. mojavensis.

KW - 16S rRNA gene

KW - Anaerobic growth

KW - Bacillus licheniformis

KW - Bacillus mojavensis

KW - Bacillus subtilis

KW - DNA-DNA similarity

KW - Phylogeny

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U2 - 10.1016/j.syapm.2006.01.010

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JO - Systematic and Applied Microbiology

JF - Systematic and Applied Microbiology

SN - 0723-2020

IS - 8

ER -

Re-identification of the halotolerant, biosurfactant-producing Bacillus licheniformis strain JF-2 as Bacillus mojavensis strain JF-2

Abstract

Keywords

ASJC Scopus subject areas

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