Re-identification of the halotolerant, biosurfactant-producing Bacillus licheniformis strain JF-2 as Bacillus mojavensis strain JF-2

Martha Folmsbee, Kathleen Duncan, Sung Ok Han, David Nagle, Eleanor Jennings, Michael McInerney

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Bacillus strain JF-2 (ATCC 39307) is a halotolerant, biosurfactant-producing bacterium that was initially described as a member of the species Bacillus licheniformis based on a limited set of phenotypic characteristics. Here, genetic and phenotypic analyses were employed to determine the relationship of Bacillus strain JF-2 to other Bacillus strains. The restriction patterns with AluI and analysis of gyrA and 16S rRNA gene sequences grouped Bacillus strain JF-2 with B. mojavensisT and not with B. licheniformisT. DNA-DNA similarity showed JF-2 was 75% similar to B. mojavensisT and only 11% similar to B. licheniformisT. Both strain JF-2 and B. mojavensisT required DNA for anaerobic growth, but B. licheniformisT did not. B. mojavensisT and strain JF-2 did not grow anaerobically in thioglycollate medium or aerobically with propionate while B. licheniformisT grew under these conditions. DNA-DNA similarity, gene sequence data and phenotypic characteristics all support the assignment of JF-2 as a member of the species B. mojavensis.

Original languageEnglish
Pages (from-to)645-649
Number of pages5
JournalSystematic and Applied Microbiology
Volume29
Issue number8
DOIs
Publication statusPublished - 2006 Dec 4
Externally publishedYes

Keywords

  • 16S rRNA gene
  • Anaerobic growth
  • Bacillus licheniformis
  • Bacillus mojavensis
  • Bacillus subtilis
  • DNA-DNA similarity
  • Phylogeny

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology

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