Abstract
Bacillus strain JF-2 (ATCC 39307) is a halotolerant, biosurfactant-producing bacterium that was initially described as a member of the species Bacillus licheniformis based on a limited set of phenotypic characteristics. Here, genetic and phenotypic analyses were employed to determine the relationship of Bacillus strain JF-2 to other Bacillus strains. The restriction patterns with AluI and analysis of gyrA and 16S rRNA gene sequences grouped Bacillus strain JF-2 with B. mojavensisT and not with B. licheniformisT. DNA-DNA similarity showed JF-2 was 75% similar to B. mojavensisT and only 11% similar to B. licheniformisT. Both strain JF-2 and B. mojavensisT required DNA for anaerobic growth, but B. licheniformisT did not. B. mojavensisT and strain JF-2 did not grow anaerobically in thioglycollate medium or aerobically with propionate while B. licheniformisT grew under these conditions. DNA-DNA similarity, gene sequence data and phenotypic characteristics all support the assignment of JF-2 as a member of the species B. mojavensis.
Original language | English |
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Pages (from-to) | 645-649 |
Number of pages | 5 |
Journal | Systematic and Applied Microbiology |
Volume | 29 |
Issue number | 8 |
DOIs | |
Publication status | Published - 2006 Dec 4 |
Externally published | Yes |
Keywords
- 16S rRNA gene
- Anaerobic growth
- Bacillus licheniformis
- Bacillus mojavensis
- Bacillus subtilis
- DNA-DNA similarity
- Phylogeny
ASJC Scopus subject areas
- Microbiology
- Ecology, Evolution, Behavior and Systematics
- Applied Microbiology and Biotechnology