Re-identification of the halotolerant, biosurfactant-producing Bacillus licheniformis strain JF-2 as Bacillus mojavensis strain JF-2

Martha Folmsbee, Kathleen Duncan, Sung Ok Han, David Nagle, Eleanor Jennings, Michael McInerney

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Bacillus strain JF-2 (ATCC 39307) is a halotolerant, biosurfactant-producing bacterium that was initially described as a member of the species Bacillus licheniformis based on a limited set of phenotypic characteristics. Here, genetic and phenotypic analyses were employed to determine the relationship of Bacillus strain JF-2 to other Bacillus strains. The restriction patterns with AluI and analysis of gyrA and 16S rRNA gene sequences grouped Bacillus strain JF-2 with B. mojavensisT and not with B. licheniformisT. DNA-DNA similarity showed JF-2 was 75% similar to B. mojavensisT and only 11% similar to B. licheniformisT. Both strain JF-2 and B. mojavensisT required DNA for anaerobic growth, but B. licheniformisT did not. B. mojavensisT and strain JF-2 did not grow anaerobically in thioglycollate medium or aerobically with propionate while B. licheniformisT grew under these conditions. DNA-DNA similarity, gene sequence data and phenotypic characteristics all support the assignment of JF-2 as a member of the species B. mojavensis.

Original languageEnglish
Pages (from-to)645-649
Number of pages5
JournalSystematic and Applied Microbiology
Volume29
Issue number8
DOIs
Publication statusPublished - 2006 Dec 4
Externally publishedYes

Fingerprint

Bacillus mojavensis
biosurfactants
Bacillus licheniformis
Bacillus
Bacillus (bacteria)
DNA
Thioglycolates
gene
Propionates
nucleotide sequences
rRNA Genes
propionates
Bacteria
bacterium
ribosomal RNA
Growth
bacteria
Genes

Keywords

  • 16S rRNA gene
  • Anaerobic growth
  • Bacillus licheniformis
  • Bacillus mojavensis
  • Bacillus subtilis
  • DNA-DNA similarity
  • Phylogeny

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Re-identification of the halotolerant, biosurfactant-producing Bacillus licheniformis strain JF-2 as Bacillus mojavensis strain JF-2. / Folmsbee, Martha; Duncan, Kathleen; Han, Sung Ok; Nagle, David; Jennings, Eleanor; McInerney, Michael.

In: Systematic and Applied Microbiology, Vol. 29, No. 8, 04.12.2006, p. 645-649.

Research output: Contribution to journalArticle

Folmsbee, Martha ; Duncan, Kathleen ; Han, Sung Ok ; Nagle, David ; Jennings, Eleanor ; McInerney, Michael. / Re-identification of the halotolerant, biosurfactant-producing Bacillus licheniformis strain JF-2 as Bacillus mojavensis strain JF-2. In: Systematic and Applied Microbiology. 2006 ; Vol. 29, No. 8. pp. 645-649.
@article{ac82cc7b5f81423b858821ebe3ca2504,
title = "Re-identification of the halotolerant, biosurfactant-producing Bacillus licheniformis strain JF-2 as Bacillus mojavensis strain JF-2",
abstract = "Bacillus strain JF-2 (ATCC 39307) is a halotolerant, biosurfactant-producing bacterium that was initially described as a member of the species Bacillus licheniformis based on a limited set of phenotypic characteristics. Here, genetic and phenotypic analyses were employed to determine the relationship of Bacillus strain JF-2 to other Bacillus strains. The restriction patterns with AluI and analysis of gyrA and 16S rRNA gene sequences grouped Bacillus strain JF-2 with B. mojavensisT and not with B. licheniformisT. DNA-DNA similarity showed JF-2 was 75{\%} similar to B. mojavensisT and only 11{\%} similar to B. licheniformisT. Both strain JF-2 and B. mojavensisT required DNA for anaerobic growth, but B. licheniformisT did not. B. mojavensisT and strain JF-2 did not grow anaerobically in thioglycollate medium or aerobically with propionate while B. licheniformisT grew under these conditions. DNA-DNA similarity, gene sequence data and phenotypic characteristics all support the assignment of JF-2 as a member of the species B. mojavensis.",
keywords = "16S rRNA gene, Anaerobic growth, Bacillus licheniformis, Bacillus mojavensis, Bacillus subtilis, DNA-DNA similarity, Phylogeny",
author = "Martha Folmsbee and Kathleen Duncan and Han, {Sung Ok} and David Nagle and Eleanor Jennings and Michael McInerney",
year = "2006",
month = "12",
day = "4",
doi = "10.1016/j.syapm.2006.01.010",
language = "English",
volume = "29",
pages = "645--649",
journal = "Systematic and Applied Microbiology",
issn = "0723-2020",
publisher = "Urban und Fischer Verlag Jena",
number = "8",

}

TY - JOUR

T1 - Re-identification of the halotolerant, biosurfactant-producing Bacillus licheniformis strain JF-2 as Bacillus mojavensis strain JF-2

AU - Folmsbee, Martha

AU - Duncan, Kathleen

AU - Han, Sung Ok

AU - Nagle, David

AU - Jennings, Eleanor

AU - McInerney, Michael

PY - 2006/12/4

Y1 - 2006/12/4

N2 - Bacillus strain JF-2 (ATCC 39307) is a halotolerant, biosurfactant-producing bacterium that was initially described as a member of the species Bacillus licheniformis based on a limited set of phenotypic characteristics. Here, genetic and phenotypic analyses were employed to determine the relationship of Bacillus strain JF-2 to other Bacillus strains. The restriction patterns with AluI and analysis of gyrA and 16S rRNA gene sequences grouped Bacillus strain JF-2 with B. mojavensisT and not with B. licheniformisT. DNA-DNA similarity showed JF-2 was 75% similar to B. mojavensisT and only 11% similar to B. licheniformisT. Both strain JF-2 and B. mojavensisT required DNA for anaerobic growth, but B. licheniformisT did not. B. mojavensisT and strain JF-2 did not grow anaerobically in thioglycollate medium or aerobically with propionate while B. licheniformisT grew under these conditions. DNA-DNA similarity, gene sequence data and phenotypic characteristics all support the assignment of JF-2 as a member of the species B. mojavensis.

AB - Bacillus strain JF-2 (ATCC 39307) is a halotolerant, biosurfactant-producing bacterium that was initially described as a member of the species Bacillus licheniformis based on a limited set of phenotypic characteristics. Here, genetic and phenotypic analyses were employed to determine the relationship of Bacillus strain JF-2 to other Bacillus strains. The restriction patterns with AluI and analysis of gyrA and 16S rRNA gene sequences grouped Bacillus strain JF-2 with B. mojavensisT and not with B. licheniformisT. DNA-DNA similarity showed JF-2 was 75% similar to B. mojavensisT and only 11% similar to B. licheniformisT. Both strain JF-2 and B. mojavensisT required DNA for anaerobic growth, but B. licheniformisT did not. B. mojavensisT and strain JF-2 did not grow anaerobically in thioglycollate medium or aerobically with propionate while B. licheniformisT grew under these conditions. DNA-DNA similarity, gene sequence data and phenotypic characteristics all support the assignment of JF-2 as a member of the species B. mojavensis.

KW - 16S rRNA gene

KW - Anaerobic growth

KW - Bacillus licheniformis

KW - Bacillus mojavensis

KW - Bacillus subtilis

KW - DNA-DNA similarity

KW - Phylogeny

UR - http://www.scopus.com/inward/record.url?scp=33751235605&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751235605&partnerID=8YFLogxK

U2 - 10.1016/j.syapm.2006.01.010

DO - 10.1016/j.syapm.2006.01.010

M3 - Article

VL - 29

SP - 645

EP - 649

JO - Systematic and Applied Microbiology

JF - Systematic and Applied Microbiology

SN - 0723-2020

IS - 8

ER -