Real-time and non-invasive optical imaging of tumor-targeting glycol chitosan nanoparticles in various tumor models

Jin Hee Na, Heebeom Koo, Sangmin Lee, Kyung Hyun Min, Kyeongsoon Park, Heon Yoo, Seung Hoon Lee, Jae Hyung Park, Ick Chan Kwon, Seo Young Jeong, Kwangmeyung Kim

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Recently, various nanoparticle systems have been developed for tumor-targeted delivery of imaging agents or drugs. However, large amount of them still have insufficient tumor accumulation and this limits their further clinical applications. Moreover, the in vivo characteristics of nanoparticles have been largely unknown, because there are few proper technologies to achieve the direct and non-invasive characterization of nanoparticles in live animals. In this paper, we determined the key factors of nanoparticles for in vivo tumor-targeting using our glycol chitosan nanoparticles (CNPs) which have proved their tumor-targeting ability in many previous papers. For this study, CNPs were labeled with near-infrared fluorescence (NIRF) dye, Cy5.5 for in vivo analysis by non-invasive optical imaging techniques. With these Cy5.5-CNPs, the factors such as in vitro/in vivo stability, deformability, and rapid uptake into target tumor cells and their effects on in vivo tumor-targeting were evaluated in various tumor-bearing mice models. In flank tumor models, Cy5.5-CNPs were selectively localized in tumor tissue than other organs, and the real-time intravascular tracking of CNPs proved the enhanced permeation and retention (EPR) effect of nanoparticles in tumor vasculature. Importantly, tumor-targeting CNPs showed an excellent tumor-specificity in brain tumors, liver tumors, and metastasis tumor models, indicating their great potential in both cancer imaging and therapy.

Original languageEnglish
Pages (from-to)5252-5261
Number of pages10
JournalBiomaterials
Volume32
Issue number22
DOIs
Publication statusPublished - 2011 Aug

Keywords

  • Brain cancer
  • Liver cancer
  • Metastasis
  • Nanoparticle
  • Tumor-targeting

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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  • Cite this

    Na, J. H., Koo, H., Lee, S., Min, K. H., Park, K., Yoo, H., Lee, S. H., Park, J. H., Kwon, I. C., Jeong, S. Y., & Kim, K. (2011). Real-time and non-invasive optical imaging of tumor-targeting glycol chitosan nanoparticles in various tumor models. Biomaterials, 32(22), 5252-5261. https://doi.org/10.1016/j.biomaterials.2011.03.076