Real time, high resolution video imaging of apoptosis in single cells with a polymeric nanoprobe

Seulki Lee, Ki Young Choi, Hyunjin Chung, Ju Hee Ryu, Aeju Lee, Heebeom Koo, In Chan Youn, Jae Hyung Park, In San Kim, Sang Yoon Kim, Xiaoyuan Chen, Seo Young Jeong, Ick Chan Kwon, Kwangmeyung Kim, Kuiwon Choi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We report a new apoptosis nanoprobe (Apo-NP) designed on the basis of a polymer nanoparticle platform. This simple one-step technique is capable of boosting fluorescence signals upon apoptosis in living cells, enabling real-time imaging of apoptosis in single cells and in vivo. The Apo-NP efficiently delivers chemically labeled, dual-quenched caspase-3-sensitive fluorogenic peptides into cells, allowing caspase-3-dependent strong fluorescence amplification to be imaged in apoptotic cells in real-time and at high resolution. The design platform of the Apo-NP is flexible and can be fine-tuned for a wide array of applications such as identification of caspase-related apoptosis in pathologies and for monitoring therapeutic efficacy of apoptotic drugs in cancer treatment.

Original languageEnglish
Pages (from-to)125-131
Number of pages7
JournalBioconjugate Chemistry
Volume22
Issue number2
DOIs
Publication statusPublished - 2011 Feb 16

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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    Lee, S., Choi, K. Y., Chung, H., Ryu, J. H., Lee, A., Koo, H., Youn, I. C., Park, J. H., Kim, I. S., Kim, S. Y., Chen, X., Jeong, S. Y., Kwon, I. C., Kim, K., & Choi, K. (2011). Real time, high resolution video imaging of apoptosis in single cells with a polymeric nanoprobe. Bioconjugate Chemistry, 22(2), 125-131. https://doi.org/10.1021/bc1004119