Real-time phase-contrast imaging of photothermal treatment of head and neck squamous cell carcinoma: An in vitro study of macrophages as a vector for the delivery of gold nanoshells

Taeseok Daniel Yang; Wonshik Choi; Tai Hyun Yoon; Kyoung Jin Lee; Jae-Seung Lee; Sang Hun Han; Min Goo Lee; Hong Soon Yim; Kyung Min Choi; Min Woo Park; Kwang-Yoon Jung; Seung Kuk Baekd

doi:10.1117/1.JBO.17.12.128003

Real-time phase-contrast imaging of photothermal treatment of head and neck squamous cell carcinoma: An in vitro study of macrophages as a vector for the delivery of gold nanoshells

Taeseok Daniel Yang, Wonshik Choi, Tai Hyun Yoon, Kyoung Jin Lee, Jae-Seung Lee, Sang Hun Han, Min Goo Lee, Hong Soon Yim, Kyung Min Choi, Min Woo Park, Kwang-Yoon Jung, Seung Kuk Baekd

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Photothermal treatment (PTT) using nanoparticles has gained attention as a promising alternative therapy for malignant tumors. One strategy for increasing the selectivity of PTT is the use of macrophages as a cellular vector for delivering nanoparticles. The aim of the present study is to examine the use of macrophages as a cellular vector for efficient PTT and determine the appropriate irradiation power and time of a near-infrared (NIR) laser using realtime phase-contrast imaging. Thermally induced injury and death of cancer cells were found to begin at 44°C to 45°C, which was achieved using the PTT effect with gold nanoshells (NS) and irradiation with a NIR laser at a power of 2 W for 5 min. The peritoneal macrophage efficiently functioned as a cellular vector for the NS, and the cancer cells surrounding the NS-loaded macrophages selectively lost their cellular viability after being irradiated with the NIR laser.

Original language	English
Article number	128003
Journal	Journal of biomedical optics
Volume	17
Issue number	12
DOIs	https://doi.org/10.1117/1.JBO.17.12.128003
Publication status	Published - 2012 Dec

Keywords

Gold nanoshells
Macrophage
Photothermal treatment
Real-time imaging
Squamous cell carcinoma

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Biomedical Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1117/1.JBO.17.12.128003

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Yang, T. D., Choi, W., Yoon, T. H., Lee, K. J., Lee, J-S., Han, S. H., Lee, M. G., Yim, H. S., Choi, K. M., Park, M. W., Jung, K-Y., & Baekd, S. K. (2012). Real-time phase-contrast imaging of photothermal treatment of head and neck squamous cell carcinoma: An in vitro study of macrophages as a vector for the delivery of gold nanoshells. Journal of biomedical optics, 17(12), [128003]. https://doi.org/10.1117/1.JBO.17.12.128003

Real-time phase-contrast imaging of photothermal treatment of head and neck squamous cell carcinoma : An in vitro study of macrophages as a vector for the delivery of gold nanoshells. / Yang, Taeseok Daniel; Choi, Wonshik ; Yoon, Tai Hyun et al.

In: Journal of biomedical optics, Vol. 17, No. 12, 128003, 12.2012.

Research output: Contribution to journal › Article › peer-review

Yang, TD, Choi, W , Yoon, TH , Lee, KJ, Lee, J-S, Han, SH, Lee, MG, Yim, HS, Choi, KM, Park, MW, Jung, K-Y & Baekd, SK 2012, 'Real-time phase-contrast imaging of photothermal treatment of head and neck squamous cell carcinoma: An in vitro study of macrophages as a vector for the delivery of gold nanoshells', Journal of biomedical optics, vol. 17, no. 12, 128003. https://doi.org/10.1117/1.JBO.17.12.128003

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abstract = "Photothermal treatment (PTT) using nanoparticles has gained attention as a promising alternative therapy for malignant tumors. One strategy for increasing the selectivity of PTT is the use of macrophages as a cellular vector for delivering nanoparticles. The aim of the present study is to examine the use of macrophages as a cellular vector for efficient PTT and determine the appropriate irradiation power and time of a near-infrared (NIR) laser using realtime phase-contrast imaging. Thermally induced injury and death of cancer cells were found to begin at 44°C to 45°C, which was achieved using the PTT effect with gold nanoshells (NS) and irradiation with a NIR laser at a power of 2 W for 5 min. The peritoneal macrophage efficiently functioned as a cellular vector for the NS, and the cancer cells surrounding the NS-loaded macrophages selectively lost their cellular viability after being irradiated with the NIR laser.",

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Real-time phase-contrast imaging of photothermal treatment of head and neck squamous cell carcinoma: An in vitro study of macrophages as a vector for the delivery of gold nanoshells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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