Real-time phase-contrast imaging of photothermal treatment of head and neck squamous cell carcinoma: An in vitro study of macrophages as a vector for the delivery of gold nanoshells

Taeseok Daniel Yang, Wonshik Choi, Tai Hyun Yoon, Kyoung Jin Lee, Jae-Seung Lee, Sang Hun Han, Min Goo Lee, Hong Soon Yim, Kyung Min Choi, Min Woo Park, Kwang-Yoon Jung, Seung Kuk Baekd

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21 Citations (Scopus)


Photothermal treatment (PTT) using nanoparticles has gained attention as a promising alternative therapy for malignant tumors. One strategy for increasing the selectivity of PTT is the use of macrophages as a cellular vector for delivering nanoparticles. The aim of the present study is to examine the use of macrophages as a cellular vector for efficient PTT and determine the appropriate irradiation power and time of a near-infrared (NIR) laser using realtime phase-contrast imaging. Thermally induced injury and death of cancer cells were found to begin at 44°C to 45°C, which was achieved using the PTT effect with gold nanoshells (NS) and irradiation with a NIR laser at a power of 2 W for 5 min. The peritoneal macrophage efficiently functioned as a cellular vector for the NS, and the cancer cells surrounding the NS-loaded macrophages selectively lost their cellular viability after being irradiated with the NIR laser.

Original languageEnglish
Article number128003
JournalJournal of Biomedical Optics
Issue number12
Publication statusPublished - 2012 Dec 1



  • Gold nanoshells
  • Macrophage
  • Photothermal treatment
  • Real-time imaging
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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