Real-time quantitative monitoring of specific peptide cleavage by a proteinase for cancer diagnosis

Gyudo Lee, Kilho Eom, Joseph Park, Jaemoon Yang, Seungjoo Haam, Yong Min Huh, Joo Kyung Ryu, Nam Hee Kim, Jong In Yook, Sang Woo Lee, Dae Sung Yoon, Taeyun Kwon

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23 Citations (Scopus)

Abstract

Good vibrations: A bioassay using a resonant peptide-functionalized microcantilever enables the quantitative characterization of the proteolytic activity of membrane type-1 matrix metalloproteinase (MT1-MMP). In this assay, shifts in the frequency of the cantilever after specific proteolytic cleavage of the target peptides by MT1-MMP are measured (see picture).

Original languageEnglish
Pages (from-to)5837-5841
Number of pages5
JournalAngewandte Chemie - International Edition
Volume51
Issue number24
DOIs
Publication statusPublished - 2012 Jun 11
Externally publishedYes

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Keywords

  • atomic force microscopy
  • biosensors
  • cancer
  • peptides
  • proteases

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

Lee, G., Eom, K., Park, J., Yang, J., Haam, S., Huh, Y. M., Ryu, J. K., Kim, N. H., Yook, J. I., Lee, S. W., Yoon, D. S., & Kwon, T. (2012). Real-time quantitative monitoring of specific peptide cleavage by a proteinase for cancer diagnosis. Angewandte Chemie - International Edition, 51(24), 5837-5841. https://doi.org/10.1002/anie.201108830