Real-time selective monitoring of allergenic Aspergillus molds using pentameric antibody-immobilized single-walled carbon nanotube-field effect transistors

Joon Hyung Jin, Junhyup Kim, Taejin Jeon, Su Kyoung Shin, Jong Ryeul Sohn, Hana Yi, Byung Yang Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Airborne fungus, including Aspergillus species, is one of the major causes of human asthma. Conventional immunoassay or DNA-sequencing techniques, although widely used, are usually labor-intensive, time-consuming and expensive. In this paper, we demonstrate a sensor for the rapid detection of Aspergillus niger, a well-known allergenic fungal species, using single-walled carbon nanotube (SWNT) field effect transistors (FETs) functionalized with pentameric antibodies that specifically bind to Aspergillus species. This strategy resulted in the real time and highly sensitive and selective detection of Aspergillus due to an electrostatic gating effect from the Aspergillus fungus. This mechanism is in contrast to a previously reported Aspergillus sensor, which was based on mobility modulation from Aspergillus adsorption. Also, our sensor shows a much wider detection range from 0.5 pg mL-1 to 10 μg mL-1 with a lower detection limit of 0.3 pg mL-1. The resulting SWNT-FET was able to selectively detect Aspergillus molds in the presence of more concentrated amounts of other mold species such as Alternaria alternata, Cladosporium cladosporioides, and Penicillium chrysogenum. We expect that our results can be used in real-time monitoring of the indoor air quality of a variety of public facilities for the elderly and children, who are more vulnerable to environmental biohazards. This journal is

Original languageEnglish
Pages (from-to)15728-15735
Number of pages8
JournalRSC Advances
Volume5
Issue number20
DOIs
Publication statusPublished - 2015

Fingerprint

Carbon nanotube field effect transistors
Immobilized Antibodies
Aspergillus
Molds
Single-walled carbon nanotubes (SWCN)
Antibodies
Monitoring
Fungi
Biohazards
Sensors
Hazardous Substances
Air quality
Electrostatics
DNA
Modulation
Personnel
Adsorption

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Real-time selective monitoring of allergenic Aspergillus molds using pentameric antibody-immobilized single-walled carbon nanotube-field effect transistors. / Jin, Joon Hyung; Kim, Junhyup; Jeon, Taejin; Shin, Su Kyoung; Sohn, Jong Ryeul; Yi, Hana; Lee, Byung Yang.

In: RSC Advances, Vol. 5, No. 20, 2015, p. 15728-15735.

Research output: Contribution to journalArticle

@article{950351e22e2a487c883d743631a3d935,
title = "Real-time selective monitoring of allergenic Aspergillus molds using pentameric antibody-immobilized single-walled carbon nanotube-field effect transistors",
abstract = "Airborne fungus, including Aspergillus species, is one of the major causes of human asthma. Conventional immunoassay or DNA-sequencing techniques, although widely used, are usually labor-intensive, time-consuming and expensive. In this paper, we demonstrate a sensor for the rapid detection of Aspergillus niger, a well-known allergenic fungal species, using single-walled carbon nanotube (SWNT) field effect transistors (FETs) functionalized with pentameric antibodies that specifically bind to Aspergillus species. This strategy resulted in the real time and highly sensitive and selective detection of Aspergillus due to an electrostatic gating effect from the Aspergillus fungus. This mechanism is in contrast to a previously reported Aspergillus sensor, which was based on mobility modulation from Aspergillus adsorption. Also, our sensor shows a much wider detection range from 0.5 pg mL-1 to 10 μg mL-1 with a lower detection limit of 0.3 pg mL-1. The resulting SWNT-FET was able to selectively detect Aspergillus molds in the presence of more concentrated amounts of other mold species such as Alternaria alternata, Cladosporium cladosporioides, and Penicillium chrysogenum. We expect that our results can be used in real-time monitoring of the indoor air quality of a variety of public facilities for the elderly and children, who are more vulnerable to environmental biohazards. This journal is",
author = "Jin, {Joon Hyung} and Junhyup Kim and Taejin Jeon and Shin, {Su Kyoung} and Sohn, {Jong Ryeul} and Hana Yi and Lee, {Byung Yang}",
year = "2015",
doi = "10.1039/c4ra15815f",
language = "English",
volume = "5",
pages = "15728--15735",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "Royal Society of Chemistry",
number = "20",

}

TY - JOUR

T1 - Real-time selective monitoring of allergenic Aspergillus molds using pentameric antibody-immobilized single-walled carbon nanotube-field effect transistors

AU - Jin, Joon Hyung

AU - Kim, Junhyup

AU - Jeon, Taejin

AU - Shin, Su Kyoung

AU - Sohn, Jong Ryeul

AU - Yi, Hana

AU - Lee, Byung Yang

PY - 2015

Y1 - 2015

N2 - Airborne fungus, including Aspergillus species, is one of the major causes of human asthma. Conventional immunoassay or DNA-sequencing techniques, although widely used, are usually labor-intensive, time-consuming and expensive. In this paper, we demonstrate a sensor for the rapid detection of Aspergillus niger, a well-known allergenic fungal species, using single-walled carbon nanotube (SWNT) field effect transistors (FETs) functionalized with pentameric antibodies that specifically bind to Aspergillus species. This strategy resulted in the real time and highly sensitive and selective detection of Aspergillus due to an electrostatic gating effect from the Aspergillus fungus. This mechanism is in contrast to a previously reported Aspergillus sensor, which was based on mobility modulation from Aspergillus adsorption. Also, our sensor shows a much wider detection range from 0.5 pg mL-1 to 10 μg mL-1 with a lower detection limit of 0.3 pg mL-1. The resulting SWNT-FET was able to selectively detect Aspergillus molds in the presence of more concentrated amounts of other mold species such as Alternaria alternata, Cladosporium cladosporioides, and Penicillium chrysogenum. We expect that our results can be used in real-time monitoring of the indoor air quality of a variety of public facilities for the elderly and children, who are more vulnerable to environmental biohazards. This journal is

AB - Airborne fungus, including Aspergillus species, is one of the major causes of human asthma. Conventional immunoassay or DNA-sequencing techniques, although widely used, are usually labor-intensive, time-consuming and expensive. In this paper, we demonstrate a sensor for the rapid detection of Aspergillus niger, a well-known allergenic fungal species, using single-walled carbon nanotube (SWNT) field effect transistors (FETs) functionalized with pentameric antibodies that specifically bind to Aspergillus species. This strategy resulted in the real time and highly sensitive and selective detection of Aspergillus due to an electrostatic gating effect from the Aspergillus fungus. This mechanism is in contrast to a previously reported Aspergillus sensor, which was based on mobility modulation from Aspergillus adsorption. Also, our sensor shows a much wider detection range from 0.5 pg mL-1 to 10 μg mL-1 with a lower detection limit of 0.3 pg mL-1. The resulting SWNT-FET was able to selectively detect Aspergillus molds in the presence of more concentrated amounts of other mold species such as Alternaria alternata, Cladosporium cladosporioides, and Penicillium chrysogenum. We expect that our results can be used in real-time monitoring of the indoor air quality of a variety of public facilities for the elderly and children, who are more vulnerable to environmental biohazards. This journal is

UR - http://www.scopus.com/inward/record.url?scp=84922563510&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922563510&partnerID=8YFLogxK

U2 - 10.1039/c4ra15815f

DO - 10.1039/c4ra15815f

M3 - Article

AN - SCOPUS:84922563510

VL - 5

SP - 15728

EP - 15735

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 20

ER -