Abstract
Real-time, sensitive and specific analysis of gene transcription: The interactions between promoter and RNA polymerase that lead to gene transcription are monitored using Rayleigh light scattering of gold nanoparticle. We can simply detect which base pair of point-mutated promoter is important for the specific recognition by RNA polymerase. This is the first attempt to analyze transcription activity, based on the optical property of single gold nanoparticle.
| Original language | English |
|---|---|
| Pages (from-to) | 1265-1269 |
| Number of pages | 5 |
| Journal | Advanced Materials |
| Volume | 25 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2013 Mar 6 |
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Keywords
- DNA-protein interaction
- gene transcription
- nanoplasmonic biosensor
- promoter activity
- real-time analysis
ASJC Scopus subject areas
- Materials Science(all)
- Mechanics of Materials
- Mechanical Engineering
Cite this
Real-time, sensitive, and specific detection of promoter-polymerase interactions in gene transcription using a nanoplasmonic sensor. / Song, Min Sun; Choi, Seung Phill; Lee, Jeewon; Kwon, Young Jik; Sim, Sang Jun.
In: Advanced Materials, Vol. 25, No. 9, 06.03.2013, p. 1265-1269.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Real-time, sensitive, and specific detection of promoter-polymerase interactions in gene transcription using a nanoplasmonic sensor
AU - Song, Min Sun
AU - Choi, Seung Phill
AU - Lee, Jeewon
AU - Kwon, Young Jik
AU - Sim, Sang Jun
PY - 2013/3/6
Y1 - 2013/3/6
N2 - Real-time, sensitive and specific analysis of gene transcription: The interactions between promoter and RNA polymerase that lead to gene transcription are monitored using Rayleigh light scattering of gold nanoparticle. We can simply detect which base pair of point-mutated promoter is important for the specific recognition by RNA polymerase. This is the first attempt to analyze transcription activity, based on the optical property of single gold nanoparticle.
AB - Real-time, sensitive and specific analysis of gene transcription: The interactions between promoter and RNA polymerase that lead to gene transcription are monitored using Rayleigh light scattering of gold nanoparticle. We can simply detect which base pair of point-mutated promoter is important for the specific recognition by RNA polymerase. This is the first attempt to analyze transcription activity, based on the optical property of single gold nanoparticle.
KW - DNA-protein interaction
KW - gene transcription
KW - nanoplasmonic biosensor
KW - promoter activity
KW - real-time analysis
UR - http://www.scopus.com/inward/record.url?scp=84874914569&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84874914569&partnerID=8YFLogxK
U2 - 10.1002/adma.201203467
DO - 10.1002/adma.201203467
M3 - Article
C2 - 23166096
AN - SCOPUS:84874914569
VL - 25
SP - 1265
EP - 1269
JO - Advanced Materials
JF - Advanced Materials
SN - 0935-9648
IS - 9
ER -