Real-time, sensitive, and specific detection of promoter-polymerase interactions in gene transcription using a nanoplasmonic sensor

Min Sun Song, Seung Phill Choi, Jeewon Lee, Young Jik Kwon, Sang Jun Sim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Real-time, sensitive and specific analysis of gene transcription: The interactions between promoter and RNA polymerase that lead to gene transcription are monitored using Rayleigh light scattering of gold nanoparticle. We can simply detect which base pair of point-mutated promoter is important for the specific recognition by RNA polymerase. This is the first attempt to analyze transcription activity, based on the optical property of single gold nanoparticle.

Original languageEnglish
Pages (from-to)1265-1269
Number of pages5
JournalAdvanced Materials
Volume25
Issue number9
DOIs
Publication statusPublished - 2013 Mar 6

Keywords

  • DNA-protein interaction
  • gene transcription
  • nanoplasmonic biosensor
  • promoter activity
  • real-time analysis

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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