Real-time, sensitive, and specific detection of promoter-polymerase interactions in gene transcription using a nanoplasmonic sensor

Min Sun Song; Seung Phill Choi; Jeewon Lee; Young Jik Kwon; Sang Jun Sim

doi:10.1002/adma.201203467

Real-time, sensitive, and specific detection of promoter-polymerase interactions in gene transcription using a nanoplasmonic sensor

Min Sun Song, Seung Phill Choi, Jeewon Lee, Young Jik Kwon, Sang Jun Sim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Real-time, sensitive and specific analysis of gene transcription: The interactions between promoter and RNA polymerase that lead to gene transcription are monitored using Rayleigh light scattering of gold nanoparticle. We can simply detect which base pair of point-mutated promoter is important for the specific recognition by RNA polymerase. This is the first attempt to analyze transcription activity, based on the optical property of single gold nanoparticle.

Original language	English
Pages (from-to)	1265-1269
Number of pages	5
Journal	Advanced Materials
Volume	25
Issue number	9
DOIs	https://doi.org/10.1002/adma.201203467
Publication status	Published - 2013 Mar 6

Keywords

DNA-protein interaction
gene transcription
nanoplasmonic biosensor
promoter activity
real-time analysis

ASJC Scopus subject areas

Materials Science(all)
Mechanics of Materials
Mechanical Engineering

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AU - Sim, Sang Jun

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