Real-time visualization of structural and biochemical information using single laser source

Ungyo Kang; Jiheun Ryu; Joon Woo Song; Jin Won Kim; Hongki Yoo; Bomi Gweon

doi:10.1117/12.2535428

Real-time visualization of structural and biochemical information using single laser source

Ungyo Kang, Jiheun Ryu, Joon Woo Song, Jin Won Kim, Hongki Yoo, Bomi Gweon

Department of Cardiology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We integrated two-photon microscopy (TPM), second harmonic imaging microscopy (SHIM), near-infrared fluorescence microscopy (NIRF), confocal laser scanning microscopy (CLSM), and fluorescence lifetime imaging microscopy (FLIM) in one microscope system. Using our microscope system, five different images were obtained simultaneously.

Original language	English
Title of host publication	Advances in Microscopic Imaging II
Editors	Emmanuel Beaurepaire, Francesco Saverio Pavone
Publisher	SPIE
ISBN (Electronic)	9781510628458
DOIs	https://doi.org/10.1117/12.2535428
Publication status	Published - 2019
Event	Advances in Microscopic Imaging II 2019 - Munich, Germany Duration: 2019 Jun 26 → 2019 Jun 27

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	11076
ISSN (Print)	1605-7422

Conference

Conference	Advances in Microscopic Imaging II 2019
Country	Germany
City	Munich
Period	19/6/26 → 19/6/27

Keywords

Confocal laser scanning microscopy
Fluorescence lifetime imaging microscopy
Multimodal optical microscope
Near infra-red fluorescence
Second harmonic generation
Two-photon microscopy

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

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Kang, U., Ryu, J., Song, J. W., Kim, J. W., Yoo, H., & Gweon, B. (2019). Real-time visualization of structural and biochemical information using single laser source. In E. Beaurepaire, & F. S. Pavone (Eds.), Advances in Microscopic Imaging II [110761Z] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11076). SPIE. https://doi.org/10.1117/12.2535428

Real-time visualization of structural and biochemical information using single laser source. / Kang, Ungyo; Ryu, Jiheun; Song, Joon Woo; Kim, Jin Won; Yoo, Hongki; Gweon, Bomi.

Advances in Microscopic Imaging II. ed. / Emmanuel Beaurepaire; Francesco Saverio Pavone. SPIE, 2019. 110761Z (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11076).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Kang, U, Ryu, J, Song, JW, Kim, JW, Yoo, H & Gweon, B 2019, Real-time visualization of structural and biochemical information using single laser source. in E Beaurepaire & FS Pavone (eds), Advances in Microscopic Imaging II., 110761Z, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11076, SPIE, Advances in Microscopic Imaging II 2019, Munich, Germany, 19/6/26. https://doi.org/10.1117/12.2535428

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title = "Real-time visualization of structural and biochemical information using single laser source",

abstract = "We integrated two-photon microscopy (TPM), second harmonic imaging microscopy (SHIM), near-infrared fluorescence microscopy (NIRF), confocal laser scanning microscopy (CLSM), and fluorescence lifetime imaging microscopy (FLIM) in one microscope system. Using our microscope system, five different images were obtained simultaneously.",

keywords = "Confocal laser scanning microscopy, Fluorescence lifetime imaging microscopy, Multimodal optical microscope, Near infra-red fluorescence, Second harmonic generation, Two-photon microscopy",

author = "Ungyo Kang and Jiheun Ryu and Song, {Joon Woo} and Kim, {Jin Won} and Hongki Yoo and Bomi Gweon",

note = "Funding Information: This work was partially supported by the National Research Foundation of Korea (NRF-2014R1A6A3A04059713).; Advances in Microscopic Imaging II 2019 ; Conference date: 26-06-2019 Through 27-06-2019",

year = "2019",

doi = "10.1117/12.2535428",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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AU - Kang, Ungyo

AU - Ryu, Jiheun

AU - Song, Joon Woo

AU - Kim, Jin Won

AU - Yoo, Hongki

AU - Gweon, Bomi

N1 - Funding Information: This work was partially supported by the National Research Foundation of Korea (NRF-2014R1A6A3A04059713).

PY - 2019

Y1 - 2019

N2 - We integrated two-photon microscopy (TPM), second harmonic imaging microscopy (SHIM), near-infrared fluorescence microscopy (NIRF), confocal laser scanning microscopy (CLSM), and fluorescence lifetime imaging microscopy (FLIM) in one microscope system. Using our microscope system, five different images were obtained simultaneously.

AB - We integrated two-photon microscopy (TPM), second harmonic imaging microscopy (SHIM), near-infrared fluorescence microscopy (NIRF), confocal laser scanning microscopy (CLSM), and fluorescence lifetime imaging microscopy (FLIM) in one microscope system. Using our microscope system, five different images were obtained simultaneously.

KW - Confocal laser scanning microscopy

KW - Fluorescence lifetime imaging microscopy

KW - Multimodal optical microscope

KW - Near infra-red fluorescence

KW - Second harmonic generation

KW - Two-photon microscopy

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DO - 10.1117/12.2535428

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T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Advances in Microscopic Imaging II

A2 - Beaurepaire, Emmanuel

A2 - Pavone, Francesco Saverio

PB - SPIE

T2 - Advances in Microscopic Imaging II 2019

Y2 - 26 June 2019 through 27 June 2019

ER -

Real-time visualization of structural and biochemical information using single laser source

Abstract

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Conference

Keywords

ASJC Scopus subject areas

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