TY - JOUR
T1 - Recent development of highly sensitive Protease assay methods
T2 - Signal amplification through enzyme cascades
AU - Yoon, Hyun Kyung
AU - Jung, Sang Taek
AU - Kim, Jae Ho
AU - Yoo, Tae Hyeon
N1 - Funding Information:
The authors acknowledge financial support from the New Faculty Research Program funded by Ajou University, the Basic Science Research Program (2012001787), and the Priority Research Centers Program (2012-0006687) through the National Research Foundation (NRF) funded by the Ministry of Education, Science and Technology.
PY - 2012
Y1 - 2012
N2 - Proteases are involved in almost all biological processes, and therefore, aberrant activity of many of these enzymes is an important indicator of disease. Various methods have been developed to analyze protease activity, among which, protease assays based on resonance energy transfer are currently used most widely. However, quantitative methods with relatively higher sensitivity are needed, especially for disease diagnosis at early stages. One of the strategies to achieve higher sensitivity is to implement signal amplification of the protease activity. In this review, we briefly summarize the protease assay methods based on resonance energy transfer, and then elaborate the efforts to develop sensitive protease assays through signal amplification by using enzyme cascades.
AB - Proteases are involved in almost all biological processes, and therefore, aberrant activity of many of these enzymes is an important indicator of disease. Various methods have been developed to analyze protease activity, among which, protease assays based on resonance energy transfer are currently used most widely. However, quantitative methods with relatively higher sensitivity are needed, especially for disease diagnosis at early stages. One of the strategies to achieve higher sensitivity is to implement signal amplification of the protease activity. In this review, we briefly summarize the protease assay methods based on resonance energy transfer, and then elaborate the efforts to develop sensitive protease assays through signal amplification by using enzyme cascades.
KW - Enzyme cascade
KW - Protease assay
KW - Resonance energy transfer
KW - Signal amplification
UR - http://www.scopus.com/inward/record.url?scp=84879924939&partnerID=8YFLogxK
U2 - 10.1007/s12257-012-0545-9
DO - 10.1007/s12257-012-0545-9
M3 - Review article
AN - SCOPUS:84879924939
SN - 1226-8372
VL - 17
SP - 1113
EP - 1119
JO - Biotechnology and Bioprocess Engineering
JF - Biotechnology and Bioprocess Engineering
IS - 6
ER -