Recent development of highly sensitive Protease assay methods: Signal amplification through enzyme cascades

Hyun Kyung Yoon, Sang Taek Jung, Jae Ho Kim, Tae Hyeon Yoo

Research output: Contribution to journalReview article

14 Citations (Scopus)

Abstract

Proteases are involved in almost all biological processes, and therefore, aberrant activity of many of these enzymes is an important indicator of disease. Various methods have been developed to analyze protease activity, among which, protease assays based on resonance energy transfer are currently used most widely. However, quantitative methods with relatively higher sensitivity are needed, especially for disease diagnosis at early stages. One of the strategies to achieve higher sensitivity is to implement signal amplification of the protease activity. In this review, we briefly summarize the protease assay methods based on resonance energy transfer, and then elaborate the efforts to develop sensitive protease assays through signal amplification by using enzyme cascades.

Original languageEnglish
Pages (from-to)1113-1119
Number of pages7
JournalBiotechnology and Bioprocess Engineering
Volume17
Issue number6
DOIs
Publication statusPublished - 2012

Keywords

  • Enzyme cascade
  • Protease assay
  • Resonance energy transfer
  • Signal amplification

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

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