Receptor-mediated delivery of all-trans-retinoic acid (ATRA) to hepatocytes from ATRA-loaded poly(N-p-vinylbenzyl-4-o-β-d-galactopyranosyl-d-gluconamide) nanoparticles

Seog Jin Seo, Hyun-Seuk Moon, Ding Ding Guo, Sang Heon Kim, Toshihiro Akaike, Chong Su Cho

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

All-trans-retinoic acid (ATRA) plays a role in regulating CYP26 gene expression in hepatocytes. Poly(N-p-vinylbenzyl-4-o-β-d-galactopyranosyl-d-gluconamide) (PVLA) nanoparticles have been used as hepatocyte-specific targeting candidates. The objective of this study was to investigate receptor-mediated delivery of ATRA using PVLA nanoparticles. ATRA-loaded PVLA nanoparticles were confirmed by 1H-nuclear magnetic resonance ( 1H-NMR) and powder X-ray diffraction (XRD). In the 1H-NMR study, the proton signals of ATRA disappeared in the spectrum of ATRA-loaded PVLA nanoparticles in D 2O, whereas in dimethylsulfoxide-d 6, the spectrum seemed like an addition of the respective spectrum of each of the pure components. The crystalline peaks of ATRA disappeared in the XRD pattern of ATRA-loaded PVLA nanoparticles after ATRA was loaded into PVLA nanoparticles. In the measurement of size distribution, diameter of PVLA and ATRA-loaded PVLA nanoparticles in aqueous solution was 6.9 and 61.2 nm in number average, respectively. Flow cytometric analysis showed that the internalization of FITC-PVLA nanoparticles by hepatocytes in the absence of a competitive inhibitor was larger than preincubated with galactose. In reverse transcription-polymerase chain reaction (RT-PCR) analysis, ATRA-loaded PVLA nanoparticles induced CYP26A1 gene in hepatocytes in the absence of a competitive inhibitor but not preincubated with galactose. The results indicate that the ATRA-loaded PVLA nanoparticle can induce CYP26A1 gene in aqueous phase by an asialoglycoprotein receptor (ASGPR)-mediated delivery system.

Original languageEnglish
Pages (from-to)136-141
Number of pages6
JournalMaterials Science and Engineering C
Volume26
Issue number1
DOIs
Publication statusPublished - 2006 Jan 1
Externally publishedYes

Fingerprint

Tretinoin
delivery
Nanoparticles
nanoparticles
acids
Acids
galactose
Galactose
genes
inhibitors
gluconamide
poly-N-4-vinylbenzyllactonamide
Hepatocytes
Genes
Nuclear magnetic resonance
Asialoglycoprotein Receptor
polymerase chain reaction
nuclear magnetic resonance
Fluorescein-5-isothiocyanate
gene expression

Keywords

  • All-trans-retinoic acid
  • Asialoglycoprotein receptor
  • CYP26A1
  • Galactose
  • Hepatocyte
  • Nanoparticle
  • Poly (N-p-vinylbenzyl-4-o-β-d-galactopyranosyl-d-gluconamide)

ASJC Scopus subject areas

  • Biomaterials

Cite this

Receptor-mediated delivery of all-trans-retinoic acid (ATRA) to hepatocytes from ATRA-loaded poly(N-p-vinylbenzyl-4-o-β-d-galactopyranosyl-d-gluconamide) nanoparticles. / Seo, Seog Jin; Moon, Hyun-Seuk; Guo, Ding Ding; Kim, Sang Heon; Akaike, Toshihiro; Cho, Chong Su.

In: Materials Science and Engineering C, Vol. 26, No. 1, 01.01.2006, p. 136-141.

Research output: Contribution to journalArticle

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abstract = "All-trans-retinoic acid (ATRA) plays a role in regulating CYP26 gene expression in hepatocytes. Poly(N-p-vinylbenzyl-4-o-β-d-galactopyranosyl-d-gluconamide) (PVLA) nanoparticles have been used as hepatocyte-specific targeting candidates. The objective of this study was to investigate receptor-mediated delivery of ATRA using PVLA nanoparticles. ATRA-loaded PVLA nanoparticles were confirmed by 1H-nuclear magnetic resonance ( 1H-NMR) and powder X-ray diffraction (XRD). In the 1H-NMR study, the proton signals of ATRA disappeared in the spectrum of ATRA-loaded PVLA nanoparticles in D 2O, whereas in dimethylsulfoxide-d 6, the spectrum seemed like an addition of the respective spectrum of each of the pure components. The crystalline peaks of ATRA disappeared in the XRD pattern of ATRA-loaded PVLA nanoparticles after ATRA was loaded into PVLA nanoparticles. In the measurement of size distribution, diameter of PVLA and ATRA-loaded PVLA nanoparticles in aqueous solution was 6.9 and 61.2 nm in number average, respectively. Flow cytometric analysis showed that the internalization of FITC-PVLA nanoparticles by hepatocytes in the absence of a competitive inhibitor was larger than preincubated with galactose. In reverse transcription-polymerase chain reaction (RT-PCR) analysis, ATRA-loaded PVLA nanoparticles induced CYP26A1 gene in hepatocytes in the absence of a competitive inhibitor but not preincubated with galactose. The results indicate that the ATRA-loaded PVLA nanoparticle can induce CYP26A1 gene in aqueous phase by an asialoglycoprotein receptor (ASGPR)-mediated delivery system.",
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