Recombinant tetra-cell adhesion motifs supports adhesion, migration and proliferation of keratinocytes/fibroblasts, and promotes wound healing

Mi Yeon Jung, Narendra Thapa, Jung Eun Kim, Duk Yang Jung, Chae Cho Byung, In-San Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

An extracellular matrix protein plays an important role in skin wound healing. In the present study, we engineered a recombinant protein encompassing the 9th and 10th type III domains of fibronectin, and 4th FAS1 domain of βig-h3. This recombinant protein, in total, harbors four known-cell adhesion motifs for integrins: Pro-His-Ser-Arg-Asn (PHSRN) and Arg-Gly-Asp (RGD) in 9th and 10th type III domains of fibronectin, respectively, and Glu-Pro-Asp-Ile-Met (EPDIM) and Try-His (YH) in 4th FAS1 domain of βig-h3, were designated to tetra-cell adhesion motifs (T-CAM). In vitro studies showed T-CAM supporting adhesion, migration and proliferation of different cell types including keratinocytes and fibroblasts. In an animal model of full-thickness skin wound, T-CAM exhibited excellent wound healing effects, superior to both 4th FAS1 domain of βig-h3 or 9th and 10th type III domains of fibronectin. Based on these results, T-CAM can be applied where enhancement of cell adhesion, migration and proliferation are desired, and it could be developed into novel wound healing drug.

Original languageEnglish
Pages (from-to)663-672
Number of pages10
JournalExperimental and Molecular Medicine
Volume39
Issue number5
DOIs
Publication statusPublished - 2007 Oct 31
Externally publishedYes

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Keywords

  • Cell adhesion
  • Cell movement
  • Cell proliferation
  • Fibronectin
  • Wound healing
  • βIG-H3 protein

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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