Recyclable Cytokines on Short and Injectable Polylactic Acid Fibers for Enhancing T-Cell Function

Hyun Mu Shin, Youngjun Ju, Gwanghun Kim, Jae Won Lee, Myung Won Seo, Ji Hyun Sim, Jusang Yang, Sora Noh, Jungbae Kim, Hang Rae Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The current practice of cytokine-based immunotherapy relies on high doses and multiple injections of cytokine agents, which raises patients' inconvenience and economic burden. Here, sustainable and recyclable cytokine delivery based on short and injectable polymer fibers immobilized with interleukin (IL)-2 and IL-15 is demonstrated, which can be retained at the target tumor sites upon injection. In detail, electrospun polylactic acid (PLA) fibers are treated with aqueous ethanol solution for their dispersion, increasing the interfiber space for highly efficient biomolecule conjugation, and further immobilized with protein G via enzymatic dopamine coating. The protein G-immobilized PLA fibers are cut into short fibers using a microtome, and filtering is performed to collect injectable short PLA (sPLA) fibers with the lengths of 15–100 µm. These sPLA fibers are further loaded with cytokines via the interaction between protein G and Fc, and cytokine-loaded sPLA (Cyto-sPLA) fibers are injected near the tumor sites using a syringe. The administration of Cyto-sPLA fibers efficiently suppresses the tumor growth up to 70% by reinvigorating nonfunctional T cells to a functional state that can kill tumors in a sustainable and recyclable manner. The injectable sPLA-fiber platform can be employed as a carrier for the efficient delivery of various agents in vivo.

Original languageEnglish
Article number1808361
JournalAdvanced Functional Materials
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

T-cells
Cytokines
acids
fibers
Acids
Fibers
Tumors
tumors
interleukins
proteins
Proteins
delivery
injection
syringes
poly(lactic acid)
Syringes
Interleukin-15
dopamine
Biomolecules
conjugation

Keywords

  • cancer immunotherapy
  • cytokine delivery
  • electrospun fibers
  • enzymatic polydopamine coating
  • injectable fibers

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Recyclable Cytokines on Short and Injectable Polylactic Acid Fibers for Enhancing T-Cell Function. / Shin, Hyun Mu; Ju, Youngjun; Kim, Gwanghun; Lee, Jae Won; Seo, Myung Won; Sim, Ji Hyun; Yang, Jusang; Noh, Sora; Kim, Jungbae; Kim, Hang Rae.

In: Advanced Functional Materials, 01.01.2019.

Research output: Contribution to journalArticle

Shin, Hyun Mu ; Ju, Youngjun ; Kim, Gwanghun ; Lee, Jae Won ; Seo, Myung Won ; Sim, Ji Hyun ; Yang, Jusang ; Noh, Sora ; Kim, Jungbae ; Kim, Hang Rae. / Recyclable Cytokines on Short and Injectable Polylactic Acid Fibers for Enhancing T-Cell Function. In: Advanced Functional Materials. 2019.
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