Red ginseng extract regulates differentiation of monocytes to macrophage and inflammatory signalings in human monocytes

Bobin Kang, Chae Young Kim, Jisu Hwang, Sojung Sun, Hyunwon Yang, Hyung Joo Suh, Hyeon Son Choi

Research output: Contribution to journalArticle

Abstract

This study was aimed to investigate the effect of red ginseng extract (RGE) on monocyte to macrophage differentiation and inflammatory signalings in THP-1 human monocytes. In HPLC analysis, RGE contained saponin level of 516 μg/mg (extract) with 14 ginsenosides. RGE effectively suppressed the monocyte-to-macrophage differentiation induced by phorbol 12-myristate 13-acetated (PMA) by inhibiting the THP-1 cell adhesion. This result is evidenced by the down-regulation of cluster of differentiation molecule β (CD11β) and CD36. RGE significantly reduced translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (78%), while cytosolic NF-κB was increased (53%), compared with LPS group. In addition, RGE significantly increased the protein abundance of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and its target protein, hemoxygenase-1 (HO-1), but, Kelch-like ECH-associated protein 1 (KEAP1), a negative regulator of Nrf2, was greatly decreased by RGE. Furthermore, RGE effectively mediated the regulation of Nrf2 level in nucleus and cytoplasm of THP-1.

Original languageEnglish
JournalFood Science and Biotechnology
DOIs
Publication statusAccepted/In press - 2019 Jan 1

Fingerprint

Panax
monocytes
Monocytes
macrophages
Macrophages
extracts
NF-E2-Related Factor 2
Ginsenosides
Saponins
nuclear proteins
Cell Adhesion
saponins
cell adhesion
Cytoplasm
B-Lymphocytes
B-lymphocytes
Down-Regulation
High Pressure Liquid Chromatography
cytoplasm
proteins

Keywords

  • Human monocyte (THP-1)
  • Inflammatory signaling
  • Monocyte-to-macrophage differentiation
  • Red ginseng extract (RGE)

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology

Cite this

Red ginseng extract regulates differentiation of monocytes to macrophage and inflammatory signalings in human monocytes. / Kang, Bobin; Kim, Chae Young; Hwang, Jisu; Sun, Sojung; Yang, Hyunwon; Suh, Hyung Joo; Choi, Hyeon Son.

In: Food Science and Biotechnology, 01.01.2019.

Research output: Contribution to journalArticle

@article{a7b28b541440431091e261743efe4711,
title = "Red ginseng extract regulates differentiation of monocytes to macrophage and inflammatory signalings in human monocytes",
abstract = "This study was aimed to investigate the effect of red ginseng extract (RGE) on monocyte to macrophage differentiation and inflammatory signalings in THP-1 human monocytes. In HPLC analysis, RGE contained saponin level of 516 μg/mg (extract) with 14 ginsenosides. RGE effectively suppressed the monocyte-to-macrophage differentiation induced by phorbol 12-myristate 13-acetated (PMA) by inhibiting the THP-1 cell adhesion. This result is evidenced by the down-regulation of cluster of differentiation molecule β (CD11β) and CD36. RGE significantly reduced translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (78{\%}), while cytosolic NF-κB was increased (53{\%}), compared with LPS group. In addition, RGE significantly increased the protein abundance of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and its target protein, hemoxygenase-1 (HO-1), but, Kelch-like ECH-associated protein 1 (KEAP1), a negative regulator of Nrf2, was greatly decreased by RGE. Furthermore, RGE effectively mediated the regulation of Nrf2 level in nucleus and cytoplasm of THP-1.",
keywords = "Human monocyte (THP-1), Inflammatory signaling, Monocyte-to-macrophage differentiation, Red ginseng extract (RGE)",
author = "Bobin Kang and Kim, {Chae Young} and Jisu Hwang and Sojung Sun and Hyunwon Yang and Suh, {Hyung Joo} and Choi, {Hyeon Son}",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/s10068-019-00611-x",
language = "English",
journal = "Food Science and Biotechnology",
issn = "1226-7708",
publisher = "Springer Netherlands",

}

TY - JOUR

T1 - Red ginseng extract regulates differentiation of monocytes to macrophage and inflammatory signalings in human monocytes

AU - Kang, Bobin

AU - Kim, Chae Young

AU - Hwang, Jisu

AU - Sun, Sojung

AU - Yang, Hyunwon

AU - Suh, Hyung Joo

AU - Choi, Hyeon Son

PY - 2019/1/1

Y1 - 2019/1/1

N2 - This study was aimed to investigate the effect of red ginseng extract (RGE) on monocyte to macrophage differentiation and inflammatory signalings in THP-1 human monocytes. In HPLC analysis, RGE contained saponin level of 516 μg/mg (extract) with 14 ginsenosides. RGE effectively suppressed the monocyte-to-macrophage differentiation induced by phorbol 12-myristate 13-acetated (PMA) by inhibiting the THP-1 cell adhesion. This result is evidenced by the down-regulation of cluster of differentiation molecule β (CD11β) and CD36. RGE significantly reduced translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (78%), while cytosolic NF-κB was increased (53%), compared with LPS group. In addition, RGE significantly increased the protein abundance of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and its target protein, hemoxygenase-1 (HO-1), but, Kelch-like ECH-associated protein 1 (KEAP1), a negative regulator of Nrf2, was greatly decreased by RGE. Furthermore, RGE effectively mediated the regulation of Nrf2 level in nucleus and cytoplasm of THP-1.

AB - This study was aimed to investigate the effect of red ginseng extract (RGE) on monocyte to macrophage differentiation and inflammatory signalings in THP-1 human monocytes. In HPLC analysis, RGE contained saponin level of 516 μg/mg (extract) with 14 ginsenosides. RGE effectively suppressed the monocyte-to-macrophage differentiation induced by phorbol 12-myristate 13-acetated (PMA) by inhibiting the THP-1 cell adhesion. This result is evidenced by the down-regulation of cluster of differentiation molecule β (CD11β) and CD36. RGE significantly reduced translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (78%), while cytosolic NF-κB was increased (53%), compared with LPS group. In addition, RGE significantly increased the protein abundance of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and its target protein, hemoxygenase-1 (HO-1), but, Kelch-like ECH-associated protein 1 (KEAP1), a negative regulator of Nrf2, was greatly decreased by RGE. Furthermore, RGE effectively mediated the regulation of Nrf2 level in nucleus and cytoplasm of THP-1.

KW - Human monocyte (THP-1)

KW - Inflammatory signaling

KW - Monocyte-to-macrophage differentiation

KW - Red ginseng extract (RGE)

UR - http://www.scopus.com/inward/record.url?scp=85071421746&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071421746&partnerID=8YFLogxK

U2 - 10.1007/s10068-019-00611-x

DO - 10.1007/s10068-019-00611-x

M3 - Article

AN - SCOPUS:85071421746

JO - Food Science and Biotechnology

JF - Food Science and Biotechnology

SN - 1226-7708

ER -