Redistribution of carbon flux toward 2,3-butanediol production in klebsiella pneumoniae by metabolic engineering

Borim Kim, Soojin Lee, Daun Jeong, Jeongmo Yang, Min-Kyu Oh, Jinwon Lee

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Klebsiella pneumoniae KCTC2242 has high potential in the production of a high-value chemical, 2,3-butanediol (2,3-BDO). However, accumulation of metabolites such as lactate during cell growth prevent large-scale production of 2,3-BDO. Consequently, we engineered K. pneumoniae to redistribute its carbon flux toward 2,3-BDO production. The ldhA gene deletion and gene overexpression (budA and budB) were conducted to block a pathway that competitively consumes reduced nicotinamide adenine dinucleotide and to redirect carbon flux toward 2,3-BDO biosynthesis, respectively. These steps allowed efficient glucose conversion to 2,3-BDO under slightly acidic conditions (pH 5.5). The engineered strain SGSB105 showed a 40% increase in 2,3-BDO production from glucose compared with that of the host strain, SGSB100. Genes closely related to 2,3-BDO biosynthesis were observed at the gene transcription level by cultivating the SGSB100, SGSB103, SGSB104, and SGSB105 strains under identical growth conditions. Transcription levels for budA, budB, and budC increased approximately 10% during the log phase of cell growth relative to that of SGSB100. Transcription levels of 2,3- BDO genes in SGSB105 remained high during the log and stationary phases. Thus, the carbon flux was redirected toward 2,3-BDO production. Data on batch culture and gene transcription provide insight into improving the metabolic network for 2,3-BDO biosynthesis for industrial applications.

Original languageEnglish
Article numbere105322
JournalPLoS One
Volume9
Issue number10
DOIs
Publication statusPublished - 2014 Oct 20

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Metabolic engineering
butanediol
Metabolic Engineering
Carbon Cycle
metabolic engineering
Klebsiella pneumoniae
Carbon
Fluxes
carbon
Genes
Transcription
Biosynthesis
transcription (genetics)
Cell growth
biosynthesis
cell growth
genes
Growth
2,3-butylene glycol
Glucose

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Redistribution of carbon flux toward 2,3-butanediol production in klebsiella pneumoniae by metabolic engineering. / Kim, Borim; Lee, Soojin; Jeong, Daun; Yang, Jeongmo; Oh, Min-Kyu; Lee, Jinwon.

In: PLoS One, Vol. 9, No. 10, e105322, 20.10.2014.

Research output: Contribution to journalArticle

Kim, Borim ; Lee, Soojin ; Jeong, Daun ; Yang, Jeongmo ; Oh, Min-Kyu ; Lee, Jinwon. / Redistribution of carbon flux toward 2,3-butanediol production in klebsiella pneumoniae by metabolic engineering. In: PLoS One. 2014 ; Vol. 9, No. 10.
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