Redox-mediated modification of PLZF by SUMO-1 and ubiquitin

Soo Im Kang, Hae Woong Choi, Ick Young Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Earlier, we reported that the transcriptional repressor promyelocytic leukemia zinc-finger protein (PLZF) is sumoylated at position K242, and the sumoylation regulated its biological function. Here, we show that the sumoylation site can be modified by ubiquitin. The stability and nuclear localization of PLZF were regulated by the antagonistic relationship between sumoylation and ubiquitination. We observed the antagonistic effects of ubiquitin and SUMO-1 on PLZF under oxidative stress induced by serum deprivation. Thus, the choice between modification of PLZF by SUMO or ubiquitin was determined by the intracellular level of ROS, which was generated by serum deprivation that inactivated the SUMO-conjugating enzymes Uba2 and Ubc9, and resulted in decrease of sumoylation. The ubiquitination was increased under these conditions. The expression of BID, a known transcriptional target protein of PLZF, was decreased, and the consequent apoptosis was induced by the ROS generated during serum starvation. On the basis of these results, we propose that PLZF post-translational modification is controlled by intracellular ROS, and the biological function of PLZF is regulated by sumoylation and ubiquitination.

Original languageEnglish
Pages (from-to)1209-1214
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume369
Issue number4
DOIs
Publication statusPublished - 2008 May 16

Fingerprint

SUMO-1 Protein
Zinc Fingers
Ubiquitin
Sumoylation
Oxidation-Reduction
Zinc
Leukemia
Ubiquitination
Proteins
Serum
Oxidative stress
Post Translational Protein Processing
Starvation
Oxidative Stress
Apoptosis

Keywords

  • PLZF
  • ROS
  • Serum deprivation
  • SUMO
  • Ubiquitin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Redox-mediated modification of PLZF by SUMO-1 and ubiquitin. / Kang, Soo Im; Choi, Hae Woong; Kim, Ick Young.

In: Biochemical and Biophysical Research Communications, Vol. 369, No. 4, 16.05.2008, p. 1209-1214.

Research output: Contribution to journalArticle

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