Reduction of Escherichia coli O157: H7 on radish seeds by sequential application of aqueous chlorine dioxide and dry-heat treatment

J. Bang, H. Kim, H. Kim, L. R. Beuchat, Y. Kim, Jee-Hoon Ryu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Aims: To assess the effectiveness of sequential treatments of radish seeds with aqueous chlorine dioxide (ClO 2) and dry heat in reducing the number of Escherichia coli O157:H7. Methods and Results: Radish seeds containing E. coli O157:H7 at 5·5 log CFUg -1 were treated with 500μgml -1 ClO 2 for 5min and subsequently heated at 60°C and 23% relative humidity for up to 48h. Escherichia coli O157:H7 decreased by more than 4·8 log CFUg -1 after 12h dry-heat treatment. The pathogen was inactivated after 48h dry-heat treatment, but the germination rate of treated seeds was substantially reduced from 91·2±5·0% to 68·7±12·3%. Conclusions: Escherichia coli O157:H7 on radish seeds can be effectively reduced by sequential treatments with ClO 2 and dry heat. To eliminate E. coli O157:H7 on radish seeds without decreasing the germination rate, partial drying of seeds at ambient temperature before dry-heat treatment should be investigated, and conditions for drying and dry-heat treatment should be optimized. Significance and Impact of the study: This study showed that sequential treatment with ClO 2 and dry-heat was effective in inactivating large numbers of E. coli O157:H7 on radish seeds. These findings will be useful when developing sanitizing strategies for seeds without compromising germination rates.

Original languageEnglish
Pages (from-to)424-429
Number of pages6
JournalLetters in Applied Microbiology
Volume53
Issue number4
DOIs
Publication statusPublished - 2011 Oct 1

Fingerprint

Raphanus
Escherichia coli O157
Seeds
Hot Temperature
Germination
chlorine dioxide
Humidity
Temperature

Keywords

  • Chlorine dioxide
  • Dry-heat treatment
  • Escherichia coli O157:H7
  • Radish seed

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Reduction of Escherichia coli O157 : H7 on radish seeds by sequential application of aqueous chlorine dioxide and dry-heat treatment. / Bang, J.; Kim, H.; Kim, H.; Beuchat, L. R.; Kim, Y.; Ryu, Jee-Hoon.

In: Letters in Applied Microbiology, Vol. 53, No. 4, 01.10.2011, p. 424-429.

Research output: Contribution to journalArticle

@article{7287b1d14f464e80ac899e1d830af238,
title = "Reduction of Escherichia coli O157: H7 on radish seeds by sequential application of aqueous chlorine dioxide and dry-heat treatment",
abstract = "Aims: To assess the effectiveness of sequential treatments of radish seeds with aqueous chlorine dioxide (ClO 2) and dry heat in reducing the number of Escherichia coli O157:H7. Methods and Results: Radish seeds containing E. coli O157:H7 at 5·5 log CFUg -1 were treated with 500μgml -1 ClO 2 for 5min and subsequently heated at 60°C and 23{\%} relative humidity for up to 48h. Escherichia coli O157:H7 decreased by more than 4·8 log CFUg -1 after 12h dry-heat treatment. The pathogen was inactivated after 48h dry-heat treatment, but the germination rate of treated seeds was substantially reduced from 91·2±5·0{\%} to 68·7±12·3{\%}. Conclusions: Escherichia coli O157:H7 on radish seeds can be effectively reduced by sequential treatments with ClO 2 and dry heat. To eliminate E. coli O157:H7 on radish seeds without decreasing the germination rate, partial drying of seeds at ambient temperature before dry-heat treatment should be investigated, and conditions for drying and dry-heat treatment should be optimized. Significance and Impact of the study: This study showed that sequential treatment with ClO 2 and dry-heat was effective in inactivating large numbers of E. coli O157:H7 on radish seeds. These findings will be useful when developing sanitizing strategies for seeds without compromising germination rates.",
keywords = "Chlorine dioxide, Dry-heat treatment, Escherichia coli O157:H7, Radish seed",
author = "J. Bang and H. Kim and H. Kim and Beuchat, {L. R.} and Y. Kim and Jee-Hoon Ryu",
year = "2011",
month = "10",
day = "1",
doi = "10.1111/j.1472-765X.2011.03125.x",
language = "English",
volume = "53",
pages = "424--429",
journal = "Letters in Applied Microbiology",
issn = "0266-8254",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Reduction of Escherichia coli O157

T2 - H7 on radish seeds by sequential application of aqueous chlorine dioxide and dry-heat treatment

AU - Bang, J.

AU - Kim, H.

AU - Kim, H.

AU - Beuchat, L. R.

AU - Kim, Y.

AU - Ryu, Jee-Hoon

PY - 2011/10/1

Y1 - 2011/10/1

N2 - Aims: To assess the effectiveness of sequential treatments of radish seeds with aqueous chlorine dioxide (ClO 2) and dry heat in reducing the number of Escherichia coli O157:H7. Methods and Results: Radish seeds containing E. coli O157:H7 at 5·5 log CFUg -1 were treated with 500μgml -1 ClO 2 for 5min and subsequently heated at 60°C and 23% relative humidity for up to 48h. Escherichia coli O157:H7 decreased by more than 4·8 log CFUg -1 after 12h dry-heat treatment. The pathogen was inactivated after 48h dry-heat treatment, but the germination rate of treated seeds was substantially reduced from 91·2±5·0% to 68·7±12·3%. Conclusions: Escherichia coli O157:H7 on radish seeds can be effectively reduced by sequential treatments with ClO 2 and dry heat. To eliminate E. coli O157:H7 on radish seeds without decreasing the germination rate, partial drying of seeds at ambient temperature before dry-heat treatment should be investigated, and conditions for drying and dry-heat treatment should be optimized. Significance and Impact of the study: This study showed that sequential treatment with ClO 2 and dry-heat was effective in inactivating large numbers of E. coli O157:H7 on radish seeds. These findings will be useful when developing sanitizing strategies for seeds without compromising germination rates.

AB - Aims: To assess the effectiveness of sequential treatments of radish seeds with aqueous chlorine dioxide (ClO 2) and dry heat in reducing the number of Escherichia coli O157:H7. Methods and Results: Radish seeds containing E. coli O157:H7 at 5·5 log CFUg -1 were treated with 500μgml -1 ClO 2 for 5min and subsequently heated at 60°C and 23% relative humidity for up to 48h. Escherichia coli O157:H7 decreased by more than 4·8 log CFUg -1 after 12h dry-heat treatment. The pathogen was inactivated after 48h dry-heat treatment, but the germination rate of treated seeds was substantially reduced from 91·2±5·0% to 68·7±12·3%. Conclusions: Escherichia coli O157:H7 on radish seeds can be effectively reduced by sequential treatments with ClO 2 and dry heat. To eliminate E. coli O157:H7 on radish seeds without decreasing the germination rate, partial drying of seeds at ambient temperature before dry-heat treatment should be investigated, and conditions for drying and dry-heat treatment should be optimized. Significance and Impact of the study: This study showed that sequential treatment with ClO 2 and dry-heat was effective in inactivating large numbers of E. coli O157:H7 on radish seeds. These findings will be useful when developing sanitizing strategies for seeds without compromising germination rates.

KW - Chlorine dioxide

KW - Dry-heat treatment

KW - Escherichia coli O157:H7

KW - Radish seed

UR - http://www.scopus.com/inward/record.url?scp=80052623487&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052623487&partnerID=8YFLogxK

U2 - 10.1111/j.1472-765X.2011.03125.x

DO - 10.1111/j.1472-765X.2011.03125.x

M3 - Article

C2 - 21770990

AN - SCOPUS:80052623487

VL - 53

SP - 424

EP - 429

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

SN - 0266-8254

IS - 4

ER -