Reduction of Escherichia coli O157:H7 on radish seeds by sequential application of aqueous chlorine dioxide and dry-heat treatment

J. Bang, H. Kim, H. Kim, L. R. Beuchat, Y. Kim, J. H. Ryu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Aims: To assess the effectiveness of sequential treatments of radish seeds with aqueous chlorine dioxide (ClO 2) and dry heat in reducing the number of Escherichia coli O157:H7. Methods and Results: Radish seeds containing E. coli O157:H7 at 5·5 log CFUg -1 were treated with 500μgml -1 ClO 2 for 5min and subsequently heated at 60°C and 23% relative humidity for up to 48h. Escherichia coli O157:H7 decreased by more than 4·8 log CFUg -1 after 12h dry-heat treatment. The pathogen was inactivated after 48h dry-heat treatment, but the germination rate of treated seeds was substantially reduced from 91·2±5·0% to 68·7±12·3%. Conclusions: Escherichia coli O157:H7 on radish seeds can be effectively reduced by sequential treatments with ClO 2 and dry heat. To eliminate E. coli O157:H7 on radish seeds without decreasing the germination rate, partial drying of seeds at ambient temperature before dry-heat treatment should be investigated, and conditions for drying and dry-heat treatment should be optimized. Significance and Impact of the study: This study showed that sequential treatment with ClO 2 and dry-heat was effective in inactivating large numbers of E. coli O157:H7 on radish seeds. These findings will be useful when developing sanitizing strategies for seeds without compromising germination rates.

Original languageEnglish
Pages (from-to)424-429
Number of pages6
JournalLetters in Applied Microbiology
Volume53
Issue number4
DOIs
Publication statusPublished - 2011 Oct

Keywords

  • Chlorine dioxide
  • Dry-heat treatment
  • Escherichia coli O157:H7
  • Radish seed

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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