Reductions catalyzed by a quinone and peroxidases from Phanerochaete chrysosporium

S. J. Rasmussen, Namhyun Chung, A. Khindaria, T. A. Grover, S. D. Aust

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A quinone produced from veratryl alcohol by lignin peroxidase from the white rot fungus Phanerochaete chrysosporium was tested for its ability to mediate reduction. The quinone (2-hydroxymethyl-5-methoxy-1,4-benzoquinone), reduced chemically or by cellobiose:quinone reductase isolated from cultures of the fungus, mediated the reduction of cytochrome c in reactions containing either Mn(III), a manganese-dependent peroxidase, Mn(II) and H 2O 2, or lignin peroxidase and H 2O 2. Formation of the semiquinone, the species responsible for reducing cytochrome c, was observed by electron spin resonance spectroscopy in these reactions. The production of the quinone was observed in the extracellular fraction of cultures grown under nutrient nitrogen-deficient conditions (2.4 mM ammonium tartrate) for over 10 days, starting on Day 2, but not under nutrient nitrogen-sufficient conditions. These results suggest that a quinone produced by lignin peroxidase can serve as a physiological mediator of reductive reactions catalyzed by the fungal peroxidases.

Original languageEnglish
Pages (from-to)243-249
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume320
Issue number2
DOIs
Publication statusPublished - 1995 Jul 26
Externally publishedYes

Fingerprint

Phanerochaete
Peroxidases
manganese peroxidase
Cytochromes c
Fungi
Nutrients
Nitrogen
Electron spin resonance spectroscopy
NAD(P)H Dehydrogenase (Quinone)
Cellobiose
Food
Electron Spin Resonance Spectroscopy
benzoquinone
lignin peroxidase

Keywords

  • peroxidase
  • quinone
  • quinone reductase
  • veratryl alcohol

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Reductions catalyzed by a quinone and peroxidases from Phanerochaete chrysosporium. / Rasmussen, S. J.; Chung, Namhyun; Khindaria, A.; Grover, T. A.; Aust, S. D.

In: Archives of Biochemistry and Biophysics, Vol. 320, No. 2, 26.07.1995, p. 243-249.

Research output: Contribution to journalArticle

Rasmussen, S. J. ; Chung, Namhyun ; Khindaria, A. ; Grover, T. A. ; Aust, S. D. / Reductions catalyzed by a quinone and peroxidases from Phanerochaete chrysosporium. In: Archives of Biochemistry and Biophysics. 1995 ; Vol. 320, No. 2. pp. 243-249.
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