Reductive activity of a manganese-dependent peroxidase from Phanerochaete chrysosporium

Namhyun Chung, M. M. Shah, T. A. Grover, S. D. Aust

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A manganese-dependent peroxidase (MnP) from Phanerochaete chrysosporium catalyzed the reduction of cytochrome c in a reaction mixture containing H2O2, Mn(II)-tartrate, and p-hydroquinone. Electron spin resonance studies have shown that the hydroquinone-dependent reductive activity of MnP is due to the benzosemiquinone formed upon the one-electron oxidation of p-hydroquinone by Mn(III)-tartrate, which is formed upon the oxidation of Mn(II) by MnP. The reductive activity increased linearly with an increase in the concentration of p-hydroquinone. The reductive activity was also observed using other hydroquinones such as methylhydroquinone, 2,5-dimethylhydroquinone, and trimethylhydroquinone. The apparent K(m) values for Mn(II) and H2O2 for the hydroquinone-dependent reductive activity were similar to those for oxidative reactions of MnP. A stoichiometry study showed that about 1.5 mol of cytochrome c was reduced per mole of H2O2 consumed. The stoichiometry decreased with an increase in the concentration of H2O2. The optimal pH for the reductive activity was 5.0, approximately the physiological pH of the fungus. The reduction of cytochrome c was also observed using a quinone and cellobiose:quinone oxidoreductase isolated from the extracellular medium of the fungus.

Original languageEnglish
Pages (from-to)70-75
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume306
Issue number1
DOIs
Publication statusPublished - 1993 Jan 1
Externally publishedYes

Fingerprint

manganese peroxidase
Phanerochaete
Cytochromes c
cellobiose-quinone oxidoreductase
Fungi
Stoichiometry
Hydroquinones
Oxidation
Electron Spin Resonance Spectroscopy
Paramagnetic resonance
hydroquinone
Electrons

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Reductive activity of a manganese-dependent peroxidase from Phanerochaete chrysosporium. / Chung, Namhyun; Shah, M. M.; Grover, T. A.; Aust, S. D.

In: Archives of Biochemistry and Biophysics, Vol. 306, No. 1, 01.01.1993, p. 70-75.

Research output: Contribution to journalArticle

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